Abstract:

BACKGROUND: With the development of science and technology and modern aerospace, the effects of mechanobiology in extreme mechanical environment—high acceleration are becoming an issue of concern. Studies have shown that high acceleration has certain effects on the cells.
OBJECTIVE: Based on a centrifuge, to design a cell loading centrifuge used for exploration of cell mechanobiology under high acceleration.
METHODS: For the cell loading centrifuge, a culture plate or/and culture bottle full of culture fluid was/were loaded with constant acceleration or variable velocity to explore the experimental feasibility. Besides, a finite element model was built by ANSYS software according to structure and properties of the rotor. The rotor system was calculated under equilibrium and dangerous working conditions, respectively, to analyze the stress and deformation distribution. Moreover, the strength of the main shaft was checked under the dangerous working conditions. Then the analysis results of ANSYS were compared with the results of strength check.
RESULTS AND CONCLUSION: Experimental findings showed that the culture plate or/and the culture bottle could be used for (0-40)×g highly constant acceleration or variable acceleration loading. Through the simulation and comparison analysis, we confirmed the reliability of the cell loading centrifuge. This cell loading centrifuge can be used to implement the study of cell mechanobiology under high acceleration in the general biology laboratory. It also provides a basis for wide application of cell loading centrifuge in the future.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Acceleration, Finite Element Analysis, Tissue Engineering