Application feasibility of xenogeneic deproteinized cancellous bone scaffold in the treatment of spinal intertransverse fusion

doi:10.3969/j.issn.2095-4344.2015.43.011

Abstract

Abstract:

BACKGROUND: Nowadays, most of the studies regarding tissue engineering bone have mostly focused on critical-size bone defects of the backbone; however, there are less studies and reports on its spinal fusion.

OBJECTIVE: To explore the feasibility of xenogeneic deproteinized cancellous bone as bone tissue engineering scaffold in the treatment of spinal intertransverse fusion.

METHODS: The cancellous part in the distal femur of adult pigs was obtained to prepare xenogeneic deproteinized cancellous bone. After combined with the recombinant human bone morphogenetic protein, the xenogeneic deproteinized cancellous bone was combined with bone marrow mesenchymal stem cells to prepare tissue engineering bone. Twenty-four goats were obtained to prepare intertransverse bone bed, and randomly divided into two groups: observation and control groups. In the observation group, the tissue engineered bone was implanted into the left side, and the xenogeneic deproteinized cancellous bone of recombinant human bone morphogenetic protein was implanted into the right side. In the control group, the autologous iliac bone was implanted into the left side, and xenogenic deproteinization cancellous bone was implanted into the right side. At the 4^th, 8^th and 12^th weeks after implantation, the fusion segment was obtained for gross observation, X-ray observation, histological observation and biomechanical testing.

RESULTS AND CONCLUSION: X-ray films showed that the implant materials from these two groups were fixed well and reliably. At different time points after implantation, the implant materials from each group were all in good position. There were no purulent and necrotic tissues around the material. Soft tissue ingrow and wraping were present. There were no effusions and necrosis surrounding the implant materials. The imaging and histological performance in the tissue engineering bone group outperformed that in the recombinant human bone morphogenetic protein xenogenic deproteinized cancellous bone group and xenogenic deproteinized cancellous bone group, which was the most close to the autogenous bone. At the 12^th week after implantation, the maximum bending load in the tissue engineering bone group was the most close to the autogenous iliac bone group. There was no significant difference between these two groups. These results demonstrate that as bone tissue engineering scaffold, xenogenic deproteinized cancellous bone has a certain application feasibility in the treatment of spinal intertransverse fusion.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Bone Transplantation, Spinal Fusion

Lv Bo, Dong Wen, Liu Wu-qing. Application feasibility of xenogeneic deproteinized cancellous bone scaffold in the treatment of spinal intertransverse fusion[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 6946-6952.

Figures/Tables 9

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