Effect of nickel-titanium wire on the expression of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor in rat myocardial cells

doi:10.3969/j.issn.2095-4344.2015.43.007

Abstract

Abstract:

BACKGROUND: Although the nickel-titanium occluder in the treatment of congenital heart disease has a better clinical effect, arrhythmia will be more likely to develop in late stage.

OBJECTIVE: To investigate the effect of nickel-titanium wire on expression of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cells.

METHODS: Thirty Sprague-Dawley rats were obtained and randomly divided into two groups: experimental and control groups. The nickel-titanium wire was implanted to the apex of heart of rats in the experimental group. Rats in the control group received no special treatment. Rat mycardial cells were harvested at the 1^th, 3^rdand 6^th months after operation. The gene and protein expressions of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cells were detected using RT-PCR and immunohistochemistry, respectively. The inflammatory reactions were detected using hematoxylin-eosin staining.

RESULTS AND CONCLUSION: After the nickel-titanium wire was implanted into the rat myocardium, inflammatory reaction was induced by inflammatory cell infiltration in the experimental group, with hyperplasia of fibrous tissue. The inflammatory reaction gradually disappeared as the implanted time extended. No inflammatory cell infiltration was visible in the control group. There was no significant difference in the gene and protein expressions of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cells at different time points after operation between these two groups. It showed that nickel-titanium wire had no influence on the expressions of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor of rat myocardial cells. These results suggest that nickel-titanium occluder-related arrhythmia may have little relationship with abnormal protein expression of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Alloys, Septal Occluder Device, Sarcoplasmic Reticulum Calcium-Transporting ATPases, Tissue Engineering

Gu Shang-wu, Jiang Qian-feng, Lu Yuan-fu, Shang Qian-hui, Wu Qin, Gong Liang, Fang Ming-liang . Effect of nickel-titanium wire on the expression of sarcoplasmic reticulum Ca~(2+)-ATPase and ryanodine receptor in rat myocardial cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 6924-6929.

Figures/Tables 5

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