Carboxymethylated chitosan effect on cyclic adenosine monophosphate/protein kinase A signaling pathway in rat Schwann cells cultured in vitro

doi:10.3969/j.issn.2095-4344.2015.43.008

Abstract

Abstract:

BACKGROUND: It has been confirmed that carboxymethylated chitosan has an promoting effect on Schwann cell proliferation and secretion, but its impact on the cyclic adenosine monophosphate-mediated protein kinase A signaling pathway in schwann cell still needs further study.

OBJECTIVE: To investigate the effect of carboxymethylated chitosan on cyclic adenosine monophosphate/ protein kinase A signaling pathway in rat schwann cells.

METHODS: The Schwann cells of the second generation neonatal rats were obtained and seeded in 6-well plate at a concentration of 1×10⁹/L. These Schwann cells were cultured and divided into four groups. The Schwann cells in the control group were cultured by adding PBS. The Schwann cells in the experimental groups were cultured by adding 50, 100 and 200 mg/L of carboxymethyl chitosan solution, respectively. After 24 hours, the concentration of cyclic adenosine monophosphate, protein kinase A activity and cyclic adenosine monophosphate response element binding protein mRNA expression were detected.

RESULTS AND CONCLUSION: Compared with the control group, carboxymethyl chitosan increased cyclic adenosine monophosphate concentrations, the activity of protein kinase A and cyclic adenosine monophosphate response element binding protein mRNA expression within the Schwann cells in a dose-dependent manner (P < 0.05). These results demonstrate that carboxymethyl chitosan can increase the concentration of cyclic adenosine monophosphate within the Schwann cells and promote protein kinase A activity, thereby activating cyclic adenosine monophosphate/protein kinase A signaling pathway.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Schwann Cells, Cell Proliferation, Cyclic AMP-Dependent Protein Kinases

He Bin, Tao Hai-ying, Wei Ai-lin, Li Xiao-hai, Chen Ren. Carboxymethylated chitosan effect on cyclic adenosine monophosphate/protein kinase A signaling pathway in rat Schwann cells cultured in vitro[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 6930-6934.

Figures/Tables 3

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