Propofol with bone marrow mesenchymal stem cell transplantation improves the hind limb function and electrophysiological changes in rats with spinal cord injury

doi:10.3969/j.issn.2095-4344.2015.41.018

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can be used to repair neurons, but have no ideal outcomes on nervous system injuries.

OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell transplantation combined with propofol on the hind limb function and electrophysiological changes of rats with spinal cord injury.

METHODS: Eighty adult Wistar rats were selected to make animal models of spinal cord injury, and then randomized into four groups (n=20): bone marrow mesenchymal stem cell group, control group, combination group, propofol group. At 6 hours after modeling, rats in these four groups were injected via the tail vein with bone marrow mesenchymal stem cell suspension, cell culture medium, bone marrow mesenchymal stem cell suspension+propofol solution, and propofol solution using a 1 mL syringe, respectively. Rat motor function was assessed by Basso Beattie Bresnahan score, modified Tarlov score and inclined plane test before and at 1 day, 3 days, 1-4 weeks after modeling. Under fluorescence microscope, the survival and distribution of PKH-26-labeled bone marrow mesenchymal stem cells were observed at 4 weeks after modeling, and meanwhile, hematoxylin-eosin staining was used for pathological observation. Horseradish peroxidase tracer analysis was performed to analyze regeneration of nerve fibers, and motor and somatosensory evoked potentials were used to analyze the neurophysiological recovery of rats.

RESULTS AND CONCLUSION: (1) The motor function of the rat hind limb recovered best in the combination group, better in the bone marrow mesenchymal stem cell group and propofol group, but worse in the control group. (2) There were a small amount of nerve axon-like structures and small syringomyelia in the bone marrow mesenchymal stem cell group and propofol group, but the combination group had more axon-like structures and no syringomyelia. In the control group, no axons but spinal cord defects and syringomyelia formed. (3) The amount of horseradish peroxidase-positive nerve fibers and the number of PKH-26 positive cells were ranked as follows: control group < propofol group and bone marrow mesenchymal stem cell group < combination group. There were significant differences between the groups (P < 0.05). (4) The latencies of motor and somatosensory evoked potentials were ranked as follows: control group> propofol group and bone marrow mesenchymal stem cell group > combination group, and there were significant differences between the groups (P < 0.05). (5) Amplitudes of motor and somatosensory evoked potentials were arranged as follows: control group < propofol group and bone marrow mesenchymal stem cell group < combination group, and the differences were statistically significant (P < 0.05). These findings indicate that both propofol and bone marrow mesenchymal stem cells can promote synaptic regeneration and improve the electrophysiological function and motor function of rats with spinal cord injury. Their combination has a better role than propofol and bone marrow mesenchymal stem cells used alone.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Propofol, Spinal Cord Injuries, Tissue Engineering

CLC Number:

R394.2

He Jue, Wang Tian-ke. Propofol with bone marrow mesenchymal stem cell transplantation improves the hind limb function and electrophysiological changes in rats with spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(41): 6659-6664.

Figures/Tables 6

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