CT perfusion-weighted imaging evaluation of neurological function recovery in cerebral infarction rats undergoing neural stem cell transplantation

doi:10.3969/j.issn.2095-4344.2015.41.017

Abstract

Abstract:

BACKGROUND: CT perfusion technology is a common non-invasive detection method, which can be used to quantitatively determine the ischemia severity and range at early stage of cerebral infarction and then judge whether ischemic brain tissues can survive or recover.
OBJECTIVE: To assess the neurological function recovery of cerebral infarction rats undergoing neural stem cell transplantation using CT perfusion imaging.
METHODS: A total of 60 Sprague-Dawley rats were randomly divided into control group, cerebral infarction group, transplantation group, with 20 rats in each group. Rat model of middle cerebral artery occlusion was made in the latter two groups. After 24 hours of modeling, PBS and 8×105 neural stem cells were administrated via the tail vein into the rats in the cerebral infarction and transplantation groups, respectively. CT perfusion-weighted imaging was performed at 1, 3, 7, 14, 28 days after transplantation. Modified neurological severity scores were recorded at 1, 2, 3, 4 weeks after transplantation. Triphenyltetrazolium chloride staining was used to calculate infarct volume at 4 weeks after transplantation. Hematoxylin- eosin staining was adopted to observe pathological changes of brain tissues at 2 weeks after transplantation.
RESULTS AND CONCLUSION: There were no abnormal hemodynamic changes in the control group at different time points. The transplantation group exhibited an increasing CT value with time, and the increased cerebral blood flow could improve the survival rate of neurons in the ischemic penumbra. The modified neurological severity score and infract volume in the transplantation group were both significantly lower than those in the cerebral infarction group (P < 0.05). Cell necrosis was improved obviously in the transplantation group. These results show that CT perfusion imaging can be used to observe the neurologic function recovery of cerebral infarction rats in aspects of morphology and hemodynamics.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Brain Infarction, Tomography, Spiral Computed, Perfusion Imaging, Neural Stem Cells, Tissue Engineering

CLC Number:

R394.2

Li Ying-xue, Gao Xue-lei. CT perfusion-weighted imaging evaluation of neurological function recovery in cerebral infarction rats undergoing neural stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(41): 6654-6658.

Figures/Tables 6

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