Neurologic function and expression of angiopoietin-1 and its receptor at recovery stage of cerebral hemorrhage after neural stem cell transplantation in rats

doi:10.3969/j.issn.2095-4344.2015.32.021

Abstract

Abstract:

BACKGROUND: Recent studies have found that bone marrow mesenchymal stem cells that cultured in vitro for a long time can naturally differentiate into neural stem cells, which then differentiate into neurons and glial cells, thereby providing a new therapeutic thinking for Parkinson’s disease, sequela of cerebral infarction, cerebellar atrophy and brain dysplasia.

OBJECTIVE: To discuss the influence of neural stem cell transplantation on neurologic function of rats with cerebral hemorrhage at recovery stage and the relevant mechanism of action.

METHODS: Sixty male Sprague-Dawley rats were randomly divided into normal group (n=18), cerebral hemorrhage group (n=21) and transplantation group (n=21). Cerebral hemorrhage models were established in the latter two groups using VII type collagen enzyme induction method. At 21 days of modeling, rats in the transplantation group were injected neural stem cells via the tail vein, and those in the other two groups received the same volume of normal saline. At 7, 14, 21 days after cell transplantation, modified adhesive removal test (MST) was employed to evaluate the neurologic function of rats, and then the rats were killed. RT-PCR was used to detect angiopoietin-1 mRNA expression in the bleeding tissues, and western blot assay was employed to measure tyrosine kinase receptor-2 protein expression.

RESULTS AND CONCLUSION: Compared with the normal group, the MST scores in the cerebral hemorrhage group and transplantation group were significantly decreased (P < 0.05). From the 7th day after transplantation, MST scores in the transplantation group were significantly higher than those in the cerebral hemorrhage group (P < 0.05). At 7, 14, 21 days after transplantation, expressions of angiopoietin-1 mRNA and tyrosine kinase receptor-2 protein were ranked as follows: transplantation group > cerebral hemorrhage group > normal group, and there was a significant difference among the three groups (P < 0.05). These findings indicate that neural stem cell transplantation can effectively promote the neurologic recovery of rats with cerebral hemorrhage at recovery stage, and the concrete mechanism may be related to the increase of angiopoietin-1 mRNA and tyrosine kinase receptor-2 protein in the bleeding tissues.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cerebral Hemorrhage, Neural Stem Cells, Angiopoietin-1, Receptor, TIE-2

CLC Number:

R394.2

Ren Hong-yu, Li Ming-xuan, He Cheng, Fan Yong-li, Ma Jian-gong, Si Hao-tian, Fang Shu-min, . Neurologic function and expression of angiopoietin-1 and its receptor at recovery stage of cerebral hemorrhage after neural stem cell transplantation in rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5199-5203.

Figures/Tables 2

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