Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (1): 118-123.doi: 10.3969/j.issn.2095-4344.2004

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Effects of cytokine-induced neutrophil chemoattractant 3 on the survival and proliferation of neural stem cells

Sun Nai1, 2, Wang Dali1, Zhang Jun3, Cao Jinming1, 2, Qiu Fucheng2, Liu Huimiao2, Li Dong2, Gu Ping2   

  1. 1Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2Department of Neurology, First Hospital of Hebei Medical University, Neuroscience Laboratory for Brain Aging and Cognition, Shijiazhuang 050031, Hebei Province, China; 3Laboratory of Cell Therapy, First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • Received:2019-03-13 Revised:2019-03-22 Accepted:2019-05-23 Online:2020-01-08 Published:2019-12-12
  • Contact: Gu Ping, Chief physician, Department of Neurology, First Hospital of Hebei Medical University, Neuroscience Laboratory for Brain Aging and Cognition, Shijiazhuang 050031, Hebei Province, China
  • About author:Sun Nai, Master, Physician, Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei Province, China; Department of Neurology, First Hospital of Hebei Medical University, Neuroscience Laboratory for Brain Aging and Cognition, Shijiazhuang 050031, Hebei Province, China Wang Dali, Chief physician, Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Supported by:
    the 2017 Key Project of Medical Science Research in Hebei Province, No. 20170062; the “Spark” Youth Research Project of First Hospital of Hebei Medical University, No. XH201702

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Abstract:

BACKGROUND: Our previous study was the first to find that bone marrow stromal cells could secrete neutrophil chemokine-3. Moreover, the expression of cytokine-induced neutrophil chemoattractant 3 (CINC-3) could be up-regulated by 1.5 times during the differentiation of neural stem cells into neurons regulated by bone marrow stromal cells. These results suggest that CINC-3 may promote the proliferation and differentiation of neural stem cells.

OBJECTIVE: To observe the effect of CINC-3 on survival and proliferation of hippocampal neural stem cells in neonatal rats.

METHODS: Hippocampal neural stem cells from neonatal Sprague-Dawley rats were isolated and cultured in vitro, and passage 3 neural stem cells were divided into control and CINC-3 groups. The survival rate of the cells was measured by MTS method, cell survival and proliferation were observed using cell growth curve and live/dead cell staining, and the expression of Nestin in neural stem cells was detected by real-time PCR and immunofluorescence staining for observation of neural stem cell proliferation.

RESULTS AND CONCLUSION: (1) When the concentration of CINC-3 increased from 1 to 20 μg/L, the survival rate of neural stem cells increased gradually. When the concentration of CINC-3 was 10 μg/L, the survival rate of neural stem cells was the highest and the cell viability was the best (P < 0.05). When the concentration of CINC-3 further increased to 20 μg/L, there was no significant difference in the survival rate of neural stem cells. (2) The positive rate of Nestin in the CINC-3 group was significantly higher than that in the control group (P < 0.05). (3) The expression of Nestin mRNA in the CINC-3 group was significantly higher than that in the control group (P < 0.05). These findings indicate CINC-3 can promote the survival and proliferation of hippocampal neural stem cells.

Key words: neutrophil chemoattractant 3, hippocampus, neural stem cells, survival, proliferation, nestin

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