Human subcutaneous adipose-derived stem cells: osteoblastic/adipogenic differentiation and identification

doi:10.3969/j.issn.2095-4344.2015.32.013

Abstract

Abstract:

BACKGROUND: Adipose-derived stem cells are a kind of mesenchyam stem cells with multipotent differentiation capacity, which have more advantages than bone marrow mesenchymal stem cells in tissue engineering research.
OBJECTIVE: To establish a method to isolate and purify adipose-derived stem cells from human subcutaneous adipose tissues followed by in vitro amplification and osteoblastic/adipogenic differentiation.
METHODS: Adipose-derived stem cells were isolated from human subcutaneous adipose tissue and cultured by density gradient centrifugation and adherent culture. Cell morphology and growth features were observed under inverted microscope. Adipose-derived stem cells at passages 2 and 5 were selected for viability measurement using cell counting kit-8 method, and then cell growth curves were drawn. The immunophenotype identification was analyzed by flow cytometry. Passage 5 cells underwent osteoblastic/adipogenic induction to confirm the multi-differentiation potential.
RESULTS AND CONCLUSION: (1) Using density gradient centrifugation and adherent culture method, high-purity human adipose-derived stem cells can be successfully isolated from human adipose tissues. (2) The growth process of human adipose-derived stem cells includes stagnant phase, logarithmic phase and plateau phase, which meets the growth rhythm of normal cells. Moreover, the population doubling time is shorter. (3).Human adipose-derived stem cells are positive for stem cell-related antigens, with low immunogenicity and the multi-differentiation potential. (4) Labeling human adipose-derived stem cells with DAPI is a simple efficient labeled method, and the labeling rate is high but the cytotoxicity is low.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: tem Cells, Subcutaneous Fat, Cell Differentiation, Cells, Cultured

CLC Number:

R394.2

Xiao Jian-hong, Zhang Yang-chun, Zhang Chang-ran, Yang Xing. Human subcutaneous adipose-derived stem cells: osteoblastic/adipogenic differentiation and identification [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5155-5161.

Figures/Tables 3

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