Biocompatibility of bio-derived xenograft bone with bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.28.006

Abstract

Abstract:

BACKGROUND: Bio-derived xenograft bone has natural pore structure of the bone, low immunogenicity, and good cytocompatibility.

OBJECTIVE: To verify the biocompatibility of bio-derived xenograft bone with bone marrow mesenchymal stem cells.

METHODS: Fresh pig femoral bone was collected to prepare bio-derived bone. Scanning electron microscope was used to observe the material structure. Passage 3 rabbit bone marrow mesenchymal stem cells at a density of 2×10⁹/L were inoculated into the cancellous bone surface of the bio-dervied bone and cultured for 7 days. Cell growth was detected using scanning electron microscope. After culture for 8 days, cell number was counted.

RESULTS AND CONCLUSION: The bio-derived bone had rough surface and irregularly interconnected pores constructing a mesh-like structure. After 3 days of compound culture, cells had irregular shapes and adhered to the surface of bio-derived bone; after 5 days of culture, cells were closely interconnected to form a layered growth; after 7 days of culture, cells exhibited multilayered growth and the extracellular matrix secreted locally. Under compound culture, the former 2 days were latent period, 3-6 days were logarithmic phase, and from the 6^th day, the cell growth curve became smooth gradually and the cell proliferation decreased and entered into the plateau period. These findings indicate that the bio-derived xenograft bone has good biocompatibility with bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Biocompatible Materials, Stem Cells, Bone Marrow

CLC Number:

R394.2

Gao Ming-jie, Tao Jie, Zhou Zi-hui, Du Lin . Biocompatibility of bio-derived xenograft bone with bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4455-4459.

Figures/Tables 1

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