Effects of curcumin on mesenchymalstem cells-induced immune tolerance to kidney transplantation

doi:10.3969/j.issn.2095-4344.2015.28.005

Abstract

Abstract:

BACKGROUND: Previous studies have shown that continuous administration of 1×10⁷ of bone marrow mesenchymal stem cells can induce immune tolerance in rats undergoing kidney transplantation, but it is not yet found clinically that curcumin exerts an on immunomodulatory effect on kidney transplantation.

OBJECTIVE: To investigate the effects of different concentrations of curcumin on bone marrow mesenchymal stem cells-induced immune tolerance in rats after kidney transplantation.

METHODS: Rat model of kidney transplantation was made, and rat models were randomly divided into four groups: transplantation group with no treatment; bone marrow mesenchymal stem cells group (cell group) injected with 1×10⁷/kg bone marrow mesenchymal stem cells via the left iliac vein (before peritoneal suture) and tail vein (from the 2^nd day) for 10 days; bone marrow mesenchymal stem cells+low/high dosage of curcumin groups (low/high dosage curcumin groups) injected intragastrically with 2 or 10 mg/kg curcumin combined with injection of bone marrow mesenchymal stem cells for 10 days. Transforming growth factor-β1 protein expression in the kidney tissues was examined by immunohistochemical staining. The concentrations of interleukin-2 and interleukin-6 in serum were detected by ELISA assay.

RESULTS AND CONCLUSION: After kidney transplantation, the protein expression of transforming growth factor-β1 in renal tubular epithelial cells and renal interstitial cells as well as the concentrations of interleukin-2 and interleukin-6 in serum were significantly higher in the transplantation group than the other groups (P < 0.05). Compared with the cell transplantation group, the protein expression of transforming growth factor-β1 as well as the concentrations of interleukin-2 and interleukin-6 reduced significantly in the low/high dosage curcumin groups (P < 0.05). These findings indicate that simultaneous administration of curcumin and bone marrow mesenchymal stem cells can effectively inhibit immune rejection reaction and improve renal function in rats after kidney transplantation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Kidney Transplantation, Interleukins

CLC Number:

R394.2

Zhang Li-li, Lv Jun, Wan Xia, Xu An-ping. Effects of curcumin on mesenchymalstem cells-induced immune tolerance to kidney transplantation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4450-4454.

Figures/Tables 3

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