Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (27): 4293-4298.doi: 10.3969/j.issn.2095-4344.2015.27.006

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Expression of early growth response factor 1 and tissue factor in rats with carotid artery injury 

Zhang Xi1, Miao Chi2
   

  1. 1 Department of Cardiology, the Second Affiliated Hospital of Shenyang Medical College, Shenyang 110001, Liaoning Province, China
    2 Department of Cardiology, the Fourth Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Zhang Xi, Department of Cardiology, the Second Affiliated Hospital of Shenyang Medical College, Shenyang 110001, Liaoning Province, China
  • About author:Zhang Xi, Department of Cardiology, the Second Affiliated Hospital of Shenyang Medical College, Shenyang 110001, Liaoning Province, China

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Abstract:

BACKGROUND: DNA enzyme targeting early growth response factor 1 (Egr-1) mRNA (ED5) can inhibit expression of downstream target genes by specifically inhibiting expression of early growth response factor 1.
OBJECTIVE: To investigate the effects of ED5 on the expression of plasma tissue factor after vascular balloon injury in rats and the mechanism of inhibiting neointimal hyperplasia.
METHODS: Intimal injury models of the left carotid artery were made in rats. Then, ED5, MgCl2 and FuGene6 were injected into the injured vascular segment.
RESULTS AND CONCLUSION: At days 3, 7, 14, 21, the expression levels of Egr-1 and tissue factor in plasma were significantly down-regulated in the ED5 transfection group compared with the MgCl2 and FuGene6 groups (P < 0.01); and neointimal hyperplasia was significantly inhibited by ED5 at days 7, 14 and 21 after modeling
(P < 0.01). ED5 may inhibit neointimal hyperplasia following balloon injury of rat common carotid artery through down-regulation of tissue factors.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Transfection, Carotid Arteries, Genes

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