Construction of nude mouse models bearing subcutaneous tumors: human osteosarcoma cell lines MG63, U2OS and 143B

doi:10.3969/j.issn.2095-4344.2015.27.003

Abstract

Abstract:

BACKGROUND: A variety of cell lines participate in the construction of animal models of osteosarcoma.
OBJECTIVE: To compare the abilities of subcutaneous tumorigenesis of human osteosarcoma cell lines MG63, U2OS and 143B in nude mice, and to establish the basis for experimental studies on osteosarcoma.
METHODS: A total of 18 nude mice were randomly divided into MG63, U2OS and 143B groups. The back of nude mice was subcutaneously injected with MG63, U2OS and 143B cell lines in corresponding groups, and the process of tumorigenesis was observed.
RESULTS AND CONCLUSION: MG63 and U2OS could not induce subcutaneous tumorigenesis in nude mice. Tumors were observed after injection with 143B cells for (6±1) days, with the tumor formation rate of 100% (6/6). The tumor grew very fast. The mean volume of tumors was (3 475±1 544) mm3 at 2 months. The survival time of nude mice burdened with tumors was (68±10) days. Hematoxylin-eosin staining revealed that in tumor tissue, cancer cells formed nest shape, nuclei were big and darkly stained, and split mostly. These results suggested that 143B cell line could well induce subcutaneous tumorigenesis, which could be used for animal research of osteosarcoma.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Osteosarcoma, Mice, Nude, Tumor Burden, Survival Analysis

CLC Number:

R318

Wu Li-mei, Wu Shao-guo, Chen Wei-wen, Zhou Lan, Zhu Jin-mei, Bao Bei. Construction of nude mouse models bearing subcutaneous tumors: human osteosarcoma cell lines MG63, U2OS and 143B[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4277-4281.

Figures/Tables 6

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