Immunohistochemical detection in dog models of spontaneous osteosarcoma

doi:10.3969/j.issn.2095-4344.0273

Abstract

Abstract:

BACKGROUND: Molecular features of osteosarcoma are not fully understood because of the complexity of osteosarcoma gene. In human osteosarcoma, there are highly expressed alkaline phosphatase, runt-related transcription factor 2 (Runx2) and bone morphogenetic protein 4 (BMP4), as well as slightly expressed desmin, S100 and neuro-specific enolase (NSE). However, whether markers of osteosarcoma are similar to those of human osteosarcoma remains unclear.
OBJECTIVE: To verify the canine osteosarcoma experiments simulating human disease model and to assess the expression of important markers for human osteosarcoma.
METHODS: Fifty-nine tissue sections of the canine osteosarcoma were used for immunohistochemical staining. The expression levels of alkaline phosphatase, desmin, S100, NSE, Runx2 and BMP4 were detected.
RESULTS AND CONCLUSION: Alkaline phosphatase, Runx2 and BMP4 were highly expressed in all tumors, while the expression of desmin, S100 and NSE was variable. The findings are similar to those found in human osteosarcoma, suggesting that canine animals can be used as the models for studying human diseases.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteosarcoma, Disease Models, Animal, Canidae, Tissue Engineering

CLC Number:

R318

Liu Yang1, Zhao De-zhi1, Xi Zheng1, Deng Zhi-hui2, Sun Qi1, Yu Shui1. Immunohistochemical detection in dog models of spontaneous osteosarcoma[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3184-3189.

Figures/Tables 1

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