Effects of self-made KYL solution on preserving macaque liver

doi:10.3969/j.issn.2095-4344.2015.24.015

Abstract

Abstract:

BACKGROUND: At present, liver transplantation is the best method to treat end-stage liver disease. UW solution is recognized as the best liver preservation solution, but it is expensive. Moreover, the extracellular fluid of high K+ condition is inconsistent with human physiology. Because transient hyperkalemia of UW solution often causes cardiac arrest, research and development of the new liver preservation solution already brook no delay.
OBJECTIVE: To study the protective effect of self-designed KYL solution on ischemia reperfusion injury in macaque donor liver.
METHODS: A total of eight recipient macaques and eight donor macaques were selected in this study. Each group contained KYL solution group (n=4) and UW solution group (n=4). Donor liver was perfused and cryopreserved for 4 hours and subjected to allogenic orthotopic liver transplantation. At 30 minutes and 6 hours after transplantation, bile production was recorded. Blood was obtained and used to detect concentrations of aspartate aminotransferase, alanine aminotransferase, nitric oxide, endothelin-1 and tumor necrosis factor-α. Liver tissue was collected and detected under the light microscope.
RESULTS AND CONCLUSION: Bile secretion was found in both groups. Bile secretion production increased as time went on (P < 0.05). At 30 minutes and 6 hours after donor liver reperfusion, serum aspartate aminotransferase and alanine aminotransferase concentrations were lower in the KYL solution group than in the UW solution group (P < 0.05). No significant difference was found in levels of serum nitric oxide, endothelin 1 and tumor necrosis factor alpha between the two groups (P > 0.05). Under light microscope, morphological observation of liver tissue revealed that cellular edema was evident in the UW solution group than in the KYL solution group. Results suggest that the effect of KYL solution on preventing ischemia/reperfusion injury was identical to the UW solution, and partial effect was better than UW solution.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Liver Transplantation, Hepatocytes

CLC Number:

R318

Bai Jian-hua, Chen Gang, Zhu Xin-feng, Zhao Xiong-qi, Liu Qi-yu, Zhao Ying-peng, Li Li. Effects of self-made KYL solution on preserving macaque liver [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3849-3854.

Figures/Tables 3

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