The expression of hepatocyte growth factor activator inhibitors 1 and 2 during liver regeneration after partial hepatectomy

doi:10.3969/j.issn.2095-4344.2015.24.014

Abstract

Abstract:

BACKGROUND: Studies have shown that the reason of the slower liver regeneration in individuals of cirrhotic liver after partial hepatectomy compared with healthy liver may be related to the delayed synthesis and secretion of hepatocyte growth factor during liver regeneration, but the cause of this phenomenon is not clear. The hepatocyte growth factor activator inhibitor found in recent years can indirectly inhibit the activation of hepatocyte growth factor, but there is little research to explore the expression of hepatocyte growth factor activator inhibitor in the regeneration process after partial hepatectomy in cirrhotic liver and its relationship with the liver regeneration.
OBJECTIVE: To investigate the expression of hepatocyte growth factor activator inhibitors (HAI-1, HAI-2) during cirrhotic and normal liver regeneration after partial hepatectomy through establishing the cirrhotic rat model, and to explore the biological effects of HAI-1, HAI-2 in cirrhotic liver during the liver regeneration after partial hepatectomy.
METHODS: We used 40% CCl4 subcutaneous injection to establish the cirrhotic rat model, then we performed 70% liver resection for the experimental group. The rats in the control group only received ordinary water feeding and 70% liver resection. Rats in each group were randomly sacrificed before surgery and at 3 hours, 6 hours, 12 hours, 24 hours and 48 hours after surgery, and samples were collected. We used RT-PCR technology to detect the expression of HAI-1 mRNA, HAI-2 mRNA in splenic tissue.
RESULTS AND CONCLUSION: The expression levels of HAI-1 mRNA of two groups after partial hepatectomy were increased firstly and then decreased. The expression of HAI-1 mRNA in cirrhotic rats was sustained higher than that of the control group (P < 0.05), there was no significant difference between the two groups of the expression of HAI-2 mRNA (P > 0.05). The expression of HAI-1 mRNA in liver cirrhosis rats after resection was consistently higher than that in healthy rats, which may lead to the insufficient synthesis and secretion of hepatocyte growth factor activator in cirrhotic rats, then hepatocyte growth factor precursor may not be activated enough, eventually leading to slow liver regeneration. HAI-2 may not be involved in the wound repair process of liver.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Yang Long1, Zhang Ya-min1, Cui Zi-lin1, Liu Zi-rong2, Yang Chao2. The expression of hepatocyte growth factor activator inhibitors 1 and 2 during liver regeneration after partial hepatectomy[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(24): 3844-3848.

Figures/Tables 1

References

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