Pulmonary perfusion for preparation of the acellular matrix

doi:10.3969/j.issn.2095-4344.2015.21.009

Abstract

Abstract:

BACKGROUND: The extracellular matrix with removal of cells and soluble proteins can maintain the normal shape of organs and matrix components.
OBJECTIVE: To prepare the acellular matrix of lung tissue using perfusion method.
METHODS: Forty Wistar rats were randomly divided into two groups: under routine anesthesia, the chest was open to obtain complete lung tissue for construction of rat lung acellular matrix scaffold using Langendorff perfusion model in experimental group, and there was no treatment in control group. Lung tissue color and shape were observed and recorded dynamically. A small tissue from different sites of the lung was taken from each group and observed histologically under electron microscope. The elastic fibers and connective tissues were highlighted by a Weigert or von Gieson staining, respectively. Hematoxylin-eosin staining was also employed.
RESULTS AND CONCLUSION: After perfusion with 1% sodium deoxycholate, the lung tissue of the experimental group gradually appeared with a piecewise and lobulated translucent appearance in white color from the inside to the outside, and the lung structure was clear. Eventually, the lung tissue became uniform white translucent. The Weigert-Von Gieson staining and hematoxylin-eosin staining showed that there were a large amount of cells in fresh lung tissue sections of the control group, including capillaries, fibroblasts and endothelial cells; the cells were arranged neatly, and had complete alveolar structure; the elastic fiber structure was clear, and the collagen fiber was arranged neatly and compactly. In the experimental group, the lung cells nearly disappeared, and the alveolar structure was still intact but in a loose state; the elastic fibers were preserved well and the collagen fibers were loosely arranged. These findings indicate that the perfusion method can be used to effectively construct the lung matrix scaffold in rats.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Lung, Extracellular Matrix

CLC Number:

R318

Gong Zheng . Pulmonary perfusion for preparation of the acellular matrix[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(21): 3329-3333.

Figures/Tables 1

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