Alveolar bone loss in rat models of diabetic periodontitis

doi:10.3969/j.issn.2095-4344.2015.05.010

Abstract

Abstract:

BACKGROUND: Body weight, blood sugar, and the change of periodontal tissue in different time period of diabetic periodontitis rats, simple chronic periodontitis rats and normal rats have different influence on the degree of alveolar bone loss.
OBJECTIVE: To establish rat models of diabetic periodontitis and chronic periodontitis, and to further clarify diabetes-increased degree of alveolar bone resorption in periodontitis rats
METHODS: Fifty-two eight-week-old specific-pathogen-free male Sprague-Dawley rats were randomly divided into diabetic periodontitis group, chronic periodontitis group and normal control group. In the diabetic periodontitis group, rat models of diabetic periodontitis were established by injection of streptozotocin + periodontal ligation. In the chronic periodontitis group, rat models of chronic periodontitis were established by periodontal ligation and constant pressure. In the normal control group, rats were fed normally. At 1, 2, 3 and 4 weeks after ligation, changes in periodontium of rats of each group were observed. The maxilla alveolar bone specimens were taken. The degree of alveolar bone loss value in rats of each group was observed under a stereomicroscope.
RESULTS AND CONCLUSION: At 1, 2, 3 and 4 weeks after ligation with steel wire, the degree of rat alveolar bone loss was as follows: diabetic periodontitis group > chronic periodontitis group > normal control group (P < 0.05). Results verified that animal models of diabetic periodontitis and chronic periodontitis were successfully established. Diabetes can increase the destruction to periodontal tissue with periodontitis, and increase alveolar bone loss.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Tissue Engineering, Chronic Periodontitis, Diabetes Mellitus, Alveolar Bone Loss

CLC Number:

R318

Wang Li-gang, Xu Bin, Dilinuer Aji, Gulinuer Awuti . Alveolar bone loss in rat models of diabetic periodontitis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 710-715.

Figures/Tables 7

References

[1] Eriksen CG, Olsen H, Husted LB, et al. The expression of IL-6 by osteoblasts is increased in healthy elderly individuals: stimulated proliferation and differentiation are unaffected by age. Calcif Tissue Int. 2010;87(5):414-423.
[2] Jurado S, Garcia-Giralt N, Díez-Pérez A, et al. Effect of IL-1beta, PGE(2), and TGF-beta1 on the expression of OPG and RANKL in normal and osteoporotic primary human osteoblasts. J Cell Biochem. 2010;110(2):304-310.
[3] Loe H. Periodontal disease The sixth complication and diabetes mellitus. Diabetes Care. 1993;16(1)329.
[4] LallaE, Lamster IB, Feit M, et al. Block ade of RAGE suppresses peri-odontitis-associated bone loss in diabetic mice. J Clin Invest. 2000;105(8):1117.
[5] Mahamed DA, Marleau A, Alnaeeli M, et al. G(-) anaerobes-reactive CD4(+) T-cells trigger RANKL-mediated enhanced alveolar bone loss in diabetic NOD mice. Diabetes. 2005;54(5):1477.
[6] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30.
[7] 郭子杰,戴永雨,栾文明,等.大鼠后牙牙槽骨吸收模型的建立及破骨细胞的鉴定[J].中国骨质疏松杂志2007,13(2):82-85
[8] 赵戬,马勇强,王宏岩,等.大鼠实验性牙周炎模型牙槽骨破坏研究[J].中国实用口腔科杂志,2012,3(5):155-158.
[9] 曹晓丽,张云,李玉明,等.糖尿病牙周炎模型大鼠的建立[J].天津医科大学学报,2010,3(9):467-469.
[10] 于静,谢景朝.实验性糖尿病牙周炎大鼠牙周组织形态结构的变化[J].疑难病杂志,2010,6(6):424-426.
[11] 张炜真,于世凤,郑麟蕃.糖尿病大鼠牙槽骨骨密度的变化[J].中华口腔医学杂志,1997,32(1):49-51.
[12] 于世凤,晓敏.牙周炎动物模型建立的技术关键[J].周病学杂志, 1999,9(1):89-91.
[13] Weinberg MA, Bral M. Laboratory animal models in periodontology. Clin Periodontol. 1999;26:335-340.
[14] Caillon P, Saffar JL. Improvement of gingival and alveolar bone status in periodontitis-affected hamsters treated with 15-methyl prostaglandin E1. Periodont Res. 1994;2(2): 138-145.
[15] Biancu S, Ericsson I, Lindhe J. Periodontal ligment tissue reaction to trauma and gingival inflammation. An experimental study in the beagledog. Clin Periodontol. 1995,22(10): 772-779.
[16] Chang KM, Ramamurthy NS. Tetracyclines inhibit porphyromonas gingivalis-induced alveolar bone loss in rats by a non-antimicrobial mechanism. J Peripdont Res. 1994; 29(4):242-249.
[17] Fischer RG, Kling B. Clinical and histological evaluation of ligature-induced periodontitis in the domestic ferret. Clin Periodont. 1994;21(4):230-239.
[18] Brunsvold MA, Chaves ES, Kornman KS, et al. effect of a bisphosphonate on experimental periodontitis in monkeys. Periodontol. 1992;63(10):825-830.
[19] Smith MA, Collins JG. Changes in inflammatory mediators in experimental periodontitis in the rhesus monkey. Infect Immun. 1993;61(4):1453-1459.
[20] 吴亚菲,赵筱芩,陈宇,等.不同方法建立大鼠实验性牙周炎模型的比较研究[J].四川大学学报,2003,34(4):742-745.
[21] Hou JC, Zemicke RF, Barnard fIJ. Experimental diabetes Insulintl eatment,and femoral neck morphology and biomechanics in rats. Clin Orthop Relat Res. 1991;(264): 278-285.
[22] Yoshida-Minami I, Suzuki A. Alveolar bone loss in rats infected with a strain of Prevotella intermedia and Fusobacterium nucleatum isolated from a child with prepubertalperiodontitis.Periodontol. 1997;68(1):12-17.
[23] 张颖丽,张莉,高文信,等.牙周炎动物模型的研究[J].白求恩医科大学学报,2001,27(6):611-613.
[24] 罗云纲,杨涛,许力强,等.牙周炎动物模型的建立[J].吉林大学学报(医学版),2004,30(2):230-232.
[25] Li J, Liu Y, Ren X, et al. Effect of oral intervention on matrix metalloproteinase-2, 9 expression in carotid arteries and serum interleukin-6 in rats with chronic periodontitis. Zhonghua Kou Qiang Yi Xue Za Zhi. 2014;49(9):554-559.
[26] Yang H, Aprecio RM, Zhou X, et al. Therapeutic effect of TSG-6 engineered iPSC-derived MSCs on experimental periodontitis in rats: a pilot study. PLoS One. 2014;9(6): e100285.
[27] Liu Y, Li J, Ren X, et al. Influence of periodontal therapy on serum interleukin-6 and carotid metalloproteinases in animals with chronic periodontitis associated with atherosclerosis. Zhonghua Kou Qiang Yi Xue Za Zhi. 2014;49(3):155-160.
[28] Ren X, Chang L, Yue Z, et al. Effects of periodontal mechanical therapy with local and systemic drugs on carotid artery and serum high-sensitivity C-reactive protein in rats with chronic periodontitis associated with atherosclerosis. Hua Xi Kou Qiang Yi Xue Za Zhi. 2013;31(5):504-508.
[29] Cavagni J, Wagner TP, Gaio EJ, et al. Obesity may increase the occurrence of spontaneous periodontal disease in Wistar rats. Arch Oral Biol. 2013;58(8):1034-1039.
[30] Fu YW, He HB. Apoptosis of periodontium cells in streptozototocin- and ligature-induced experimental diabetic periodontitis in rats. Acta Odontol Scand. 2013;71(5): 1206-1215.
[31] Verzeletti GN, Gaio EJ, Linhares DS, et al. Effect of obesity on alveolar bone loss in experimental periodontitis in Wistar rats. J Appl Oral Sci. 2012;20(2):218-221.
[32] Lütfio?lu M, Sakallio?lu U, Sakallio?lu EE, et al. The impact of dietary induced hyperparathyroidism on healthy and diseased periodontia: an experimental study in rats. J Clin Periodontol. 2012;39(3):264-271.
[33] Tokudome Y, Ito A, Otsuka M. Effect of zinc-containing β-tricalcium phosphate nano particles injection on jawbone mineral density and mechanical strength of osteoporosis model rats. Biol Pharm Bull. 2011;34(8):1215-1218.
[34] Zhang DX, Li M, Pang BS, et al. Establishment of chronic periodontitis and chronic obstructive pulmonary disease model in SD rat. Zhonghua Kou Qiang Yi Xue Za Zhi. 2010; 45(11):668-672.