Expression of repulsive guidance molecule A in the hippocampus of rat models of cerebral ischemia-reperfusion injury during treadmill exercise

doi:10.3969/j.issn.2095-4344.2015.05.006

Abstract

Abstract:

BACKGROUND: Exercises play an important role in the recovery of neurological function after stroke. Few studies concerned the amount of exercise in rats after stroke. Hippocampus is strongly associated with cognitive function, but no reports addressed the expression of repulsive guidance molecule A in the rat hippocampus after ischemia and reperfusion.
OBJECTIVE: To explore the effects of exercise on repulsive guidance molecule A expression in the hippocampus on the ischemic side in rats after cerebral ischemia-reperfusion injury.
METHODS: 120 Sprague-Dawley rats were equally and randomly divided into normal group, sham-operation group, and 7-, 14-, 28-day model groups. The model of right cerebral ischemia-reperfusion injury was induced by ligation with nylon monofilament in rats of 7-, 14-, 28-day model groups. Low exercise group received treadmill training of 5 m/min, 5 minutes; 7 m/min, 5 minutes; 9 m/min, 20 minutes. Moderate exercise group received treadmill training of 8 m/min, 5 minutes; 10 m/min, 5 minutes; 13 m/min, 20 minutes. High exercise group received treadmill training of 8 m/min, 5 minutes; 11 m/min, 5 minutes; 20 m/min, 20 minutes.
RESULTS AND CONCLUSION: Repulsive guidance molecule A mRNA and protein expression was highest in the ischemic side of the hippocampus in the 7-day model group without excercise. Moreover, repulsive guidance molecule A relative expression gradually reduced over time. Compared with non-exercise, repulsive guidance molecule A mRNA and protein expression significantly decreased in the 14- and 28-day model groups during moderate exercise (P < 0.05), but repulsive guidance molecule A mRNA and protein expression increased during high exercise. Above data confirmed that moderate exercises could decrease repulsive guidance molecule A expression in the affected side of the hippocampus of rats with cerebral ischemia-reperfusion injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Reperfusion Injury, Sports, Hippocampus, Motor Activity

CLC Number:

R318

Guo Zhen-wei, Qin Xin-yue, Zhang Guang-hui. Expression of repulsive guidance molecule A in the hippocampus of rat models of cerebral ischemia-reperfusion injury during treadmill exercise[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 685-690.

Figures/Tables 2

References

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