Hydrogen peroxide influences embryonic stem cell transplantation in the treatment of myocardial infarction

doi:10.3969/j.issn.2095-4344.2015.01.001

Abstract

Abstract:

BACKGROUND: Specific mechanism of stem cell transplantation for treatment of myocardial infarction has been not very clear.
OBJECTIVE: To investigate the effect of H₂O₂-pretreated mouse embryonic stem cells in vitro for treatment of myocardial infarction.
METHODS: Twenty-four C57BL/6 mice, 9 weeks old, were randomized into myocardial infarction group, embryonic stem cells group, H₂O₂ pretreatment group, with eight mice in each group, which were respectively injected 150 μL PBS, 150 μL embryonic stem cells (1×10⁶), and 150 μL H₂O₂-pretreated embryonic stem cells (1×10⁶) at 1 week after modeling. At 3 weeks after transplantation, left ventricular end-diastolic diameter, left ventricular end-systolic inner diameter and left ventricular ejection fraction of mice in each group were measured by ultrasound. Myocardial collagen content was detected by Sirius red staining and the average collagen volume fraction was calculated.
RESULTS AND CONCLUSION: Compared with the myocardial infarction group, the left ventricular end-diastolic diameter and left ventricular end-systolic inner diameter were significantly decreased in the embryonic stem cells group and H₂O₂ pretreatment group, while the left ventricular ejection fraction was significantly increased (P < 0.05). Heart weight/body weight, left ventricular weight/body weight, and average collagen volume fraction were significantly lower in the embryonic stem cells group and H₂O₂ pretreatment group than the myocardial infarction group (P < 0.05). Therefore, in vitro H₂O₂ pretreatment enhances reduce the post-myocardial infarction myocardial fibrosis and greatly improve heart function.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Embryonic Stem Cells, Hydrogen Peroxide, Myocardial Infarction, Interleukin-6

CLC Number:

R394.2

Fan Zhi-gang, Kong Chun-yan. Hydrogen peroxide influences embryonic stem cell transplantation in the treatment of myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 1-6.

Figures/Tables 5

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