Protein extracts from human embryonic brain tissue induce the neuronal differentiation of mesenchymal stem cells from human placenta

doi:10.3969/j.issn.2095-4344.2014.50.023

Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (50): 8168-8173.doi: 10.3969/j.issn.2095-4344.2014.50.023

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Protein extracts from human embryonic brain tissue induce the neuronal differentiation of mesenchymal stem cells from human placenta

Ma Li-jun¹, Fan Heng¹, Ma Xiao-na¹, Wei Jun^{1, 2}, Li Yu-kui^{1, 2}

1Ningxia Human Stem Cell Institute, the General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; 2Province-Ministry Co-Constructed Key Laboratory of Fecundity Preserve, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China

Online:2014-12-03 Published:2014-12-03
Contact: Wei Jun, Master, Professor, Ningxia Human Stem Cell Institute, the General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Province-Ministry Co-Constructed Key Laboratory of Fecundity Preserve, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China Corresponding author: Li Yu-kui, M.D., Professor, Ningxia Human Stem Cell Institute, the General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China; Province-Ministry Co-Constructed Key Laboratory of Fecundity Preserve, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
About author:Ma Li-jun, Studying for doctorate, Assistant researcher, Ningxia Human Stem Cell Institute, the General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
Supported by:
the Natural Science Foundation of Ningxia Hui Autonomous Region, No. NZ1229; the Project of Ningxia Medical University, No. XM201135

Abstract

Abstract:

BACKGROUND: It is a key to choose an appropriate method to trans-differentiate mesenchymal stem cells from human placenta into neuron-like cells for clinical treatment of neural system injury.
OBJECTIVE: To observe the feasibility of the neuronal differentiation of mesenchymal stem cells from human placenta with protein extracts of human embryonic brain tissue.
METHODS: Mesenchymal stem cells from human placenta were isolated and cultured using enzyme digestion method. The third passage of cells were incubated in induction medium containing protein extracts of human embryonic brain tissue for 6 days, and then cultured in culture medium containing 20 nmol/L all-trans retinoic acid and 500 μg/L sonic hedgehog for 3 days, followed by 3-day continuous culture in 10 μg/L brain-derived neurotrophic factor, 20 μg/L glial cell line-derived neurotrophic factor, 50 μg/L insulin-like growth factor type II
RESULTS AND CONCLUSION: The adherent cells were obtained after enzyme digestion of placenta tissue. The passage 2 cells were fusiform-shaped and exhibited a whirlpool-like growth. The passage 3 cells were uniform in
morphology. After induction, immunocytochemistry assay showed that the cells expressed neuron-specific enolase, nestin, glial fibrillary acidic protein, neurofilament protein, and nerve growth associated protein 43; ELISA results showed that cells were positive for brain-derived neurotrophic factor, neurotrophin 3, neurotrophin 4; RT-PCR results showed that cells were positive for microtubule-associated protein, neuron-specific enolase, neurofilament protein, nerve growth associated protein 43. Results indicate that mesenchymal stem cells from human placenta can differentiate into neuron-like cells under induction of protein extracts of human embryonic brain tissue.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: placenta, mesenchymal stem cells, neurons, embryonic induction, cell differentiation

CLC Number:

R394.2

Ma Li-jun, Fan Heng, Ma Xiao-na, Wei Jun, Li Yu-kui. Protein extracts from human embryonic brain tissue induce the neuronal differentiation of mesenchymal stem cells from human placenta[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8168-8173.

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References

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Protein extracts from human embryonic brain tissue induce the neuronal differentiation of mesenchymal stem cells from human placenta

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