MHC molecule expression in dental pulp stem cells and mixed lymphocyte proliferation in vitro

doi:10.3969/j.issn.2095-4344.2014.50.022

Abstract

Abstract:

BACKGROUND: If differentiated dental pulp stem cells have immune adjustment ability similar to undifferentiated ones, they could become a new source of allogeneic seed cells in tissue engineering.
OBJECTIVE: To study the immunological properties of dental pulp stem cells and their immunomodulatory effects on lymphocytes in vitro.
METHODS: Dental pulp stem cells were isolated from mouse dental pulp tissue. Cells at passage 2 were detected using flow cytometry for the expression of MHC-I, MHC-II. Inguinal lymphcells were cultured with allogeneic dental pulp stem cells at a density of 1×105 cells per well. Dental pulp stem cells at 1×105 cells per well or interferon-γ-treated dental pulp stem cells were added into the mixed lymphocyte reaction system to observe lymphocyte proliferation.
RESULTS AND CONCLUSION: Flow cytometry showed that undifferentiated dental pulp stem cells expressed MHC-I but not MHC-II. After intervention with interferon-γ for 48 hours, increased expression of MHC-I could not been seen in the dental pulp stem cells, but MHC-II expression was increased obviously. No lymphocyte response was induced by allogeneic or interferon-γ-treated dental pulp stem cells as stimulators. Experimental findings suggest that dental pulp stem cells can modulate the proliferation of lymphocytes, which may be expected to become a new source of allogeneic seed cells in tissue engineering or cell therapy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, dental pulp, lymphocyte culture test, mixed

CLC Number:

R394.2

Nie Shan-shan, Liu Jia, Zhang Rui-han, Wang Xuan, Li Bo-qi, Sun Da-lei, Refukati, Liu Yi-shan. MHC molecule expression in dental pulp stem cells and mixed lymphocyte proliferation in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8162-8167.

Figures/Tables 2

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