MicroRNA-21 can promote the differentiation of neural crest stem cells from human follicle into Schwann cells

doi:10.3969/j.issn.2095-4344.2014.50.021

Abstract

Abstract:

BACKGROUND: MicroRNAs are a class of non-coding single-stranded small RNA molecules containing 18–25 nucleotides that can bind to the 3’UTR of the mRNA molecules and regulate the protein expression of target genes. Studies have shown that microRNAs could regulate Schwann cell differentiation, myelination maturation and growth of the peripheral nerve.
OBJECTIVE: To observe the expression of miR-21 during the differentiation of neural crest stem cells from human follicle into Schwann cells.
METHODS: Hair follicle stem cells were cultured and neural crest stem cells were separated from human hair follicles by flow cytometry. Then, the neural crest stem cells were induced to differentiate into Schwann cells.
qRT-PCR was used to detect the expression of miR-21 in the process of induction. Neural crest stem cells from hair follicles were divided into control group, agomir-21 group, agomir-NC group, antagomir-21 group and antagomir-NC group. The control group was without intervention. Agomir-21 group was transfected with miR-21 agonist, whereas Antagomir-21 group was transfected with miR-21 antagonist. agomir-NC group and antagomir-NC group were respectively negative controls of agomir-21 group and antagomir-21 group. Finally, the possible target of miR-21 was searched in database.
RESULTS AND CONCLUSION: Neural crest stem cells were successfully separated from human hair follicles using flow cytometry and induced to differentiate into Schwann cells. In the process of cell differentiation, miR-21 expression was upregulated gradually. Transfection of miR-21 agonist could enhance the stem cell differentiation into Schwann cells, whereas transfection of miR-21 antagonist could weaken the differentiation capacity of stem cells. Furthermore, we found via database searching that SOX2 maybe a target of miR-21 and participate in the regulatory role of miR-21. This study suggested that hair follicle neural crest stem cells can be used as an important source of Schwann cells and miR-21 can promote the differentiation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, neural crest, hair follicle, Schwann cells, microRNAs

CLC Number:

R394.2

Wang Yan-hua, Liu Hao, Xin Hong, Bai Xiao-xue, Liu Xue-juan, Ni Yu-xin. MicroRNA-21 can promote the differentiation of neural crest stem cells from human follicle into Schwann cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8156-8161.

Figures/Tables 2

References

[1] Sieber-Blum M, Grim M.The adult hair follicle: cradle for pluripotent neural crest stem cells.Birth Defects Res C Embryo Today. 2004;72(2):162-172.
[2] Liu X, Song L, Liu J,et al.miR-18b inhibits TGF-β1-induced differentiation of hair follicle stem cells into smooth muscle cells by targeting SMAD2.Biochem Biophys Res Commun. 2013;438(3):551-556.
[3] Lin H, Liu F, Zhang C,et al.Characterization of nerve conduits seeded with neurons and Schwann cells derived from hair follicle neural crest stem cells.Tissue Eng Part A. 2011; 17(13-14):1691-1698.
[4] Lin H, Liu F, Zhang C,et al.Pluripotent hair follicle neural crest stem-cell-derived neurons and schwann cells functionally repair sciatic nerves in rats.Mol Neurobiol. 2009;40(3): 216-223.
[5] Dugas JC, Notterpek L.MicroRNAs in oligodendrocyte and Schwann cell differentiation.Dev Neurosci. 2011;33(1):14-20.
[6] Yu B, Qian T, Wang Y,et al.miR-182 inhibits Schwann cell proliferation and migration by targeting FGF9 and NTM, respectively at an early stage following sciatic nerve injury. Nucleic Acids Res. 2012;40(20):10356-10365.
[7] Yu B, Zhou S, Wang Y,et al.miR-221 and miR-222 promote Schwann cell proliferation and migration by targeting LASS2 after sciatic nerve injury.J Cell Sci. 2012;125(Pt 11):2675- 2683.
[8] Gokey NG, Srinivasan R, Lopez-Anido C, et al.Developmental regulation of microRNA expression in Schwann cells.Mol Cell Biol. 2012;32(2):558-568.
[9] Yu H, Kumar SM, Kossenkov AV,et al.Stem cells with neural crest characteristics derived from the bulge region of cultured human hair follicles.J Invest Dermatol. 2010;130(5):1227- 1236.
[10] Krej?í E, Grim M.Isolation and characterization of neural crest stem cells from adult human hair follicles.Folia Biol (Praha). 2010;56(4):149-157.
[11] Jiang X, Gwye Y, McKeown SJ,et al.Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells.Stem Cells Dev. 2009;18(7):1059-1070.
[12] Yang R, Xu X.Isolation and culture of neural crest stem cells from human hair follicles.J Vis Exp. 2013;(74). doi: 10.3791/3194.
[13] Wu WF. Follicle and melanocyte stem cells, and their application in neuroscience A Web of Science-based literature analysis. Neural Regen Res. 2012; 7(34): 2734-2741.
[14] Liu Q, Spusta SC, Mi R,et al.Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells.Stem Cells Transl Med. 2012;1(4):266-278.
[15] Kumarswamy R, Volkmann I, Thum T.Regulation and function of miRNA-21 in health and disease.RNA Biol. 2011;8(5): 706-713.
[16] Iliopoulos D, Jaeger SA, Hirsch HA,et al.STAT3 activation of miR-21 and miR-181b-1 via PTEN and CYLD are part of the epigenetic switch linking inflammation to cancer.Mol Cell. 2010;39(4):493-506.
[17] Bornachea O, Santos M, Martínez-Cruz AB,et al.EMT and induction of miR-21 mediate metastasis development in Trp53-deficient tumours.Sci Rep. 2012;2:434.
[18] Liu J, Kern JA.Neuregulin-1 activates the JAK-STAT pathway and regulates lung epithelial cell proliferation.Am J Respir Cell Mol Biol. 2002;27(3):306-313.
[19] Le N, Nagarajan R, Wang JY,et al.Analysis of congenital hypomyelinating Egr2Lo/Lo nerves identifies Sox2 as an inhibitor of Schwann cell differentiation and myelination.Proc Natl Acad Sci U S A. 2005;102(7):2596-2601.
[20] Adameyko I, Lallemend F, Furlan A,et al.Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest.Development. 2012;139(2):397-410