Adipogenic and tenogenic differentiation of tendon-derived stem cells isolated from an animal model of chronic tendinopathy in vitro

doi:10.3969/j.issn.2095-4344.2014.45.019

Abstract

Abstract:

BACKGROUND: Chronic tendinopathy is a tendon disorder extremely common in athletes and in the general population with repetitive strain injuries of tendons. The pathogenesis of tendinopathy remains unclear and hence treatment of tendinopathy is usually palliative.

OBJECTIVE: To investigate the of adipogenic and tenogenic ability of patellar tendon-derived stem cells isolated from chronic tendinopathy and healthy rats in vitro.

METHODS:Tendon-derived stem cells were isolated from patellar tendons of chronic tendinopathy and healthy rats respectively. The tendon-derived stem cells were cultured to the 3^rd passage in complete culture medium, and cell morphology was observed. The cells were divided into adipogenic induction group and control group. Cells in the adipogenic induction group were cultured in adipogenic induction medium, while those in the control group cultured in complete culture medium. The ability of adipogenic differentiation between tendon-derived stem cells isolated from the tendon of chronic tendinopathy and healthy rats in vitro was examined by oil red O staining and quantification assay. The mRNA expressions of C/EBPα and PPARγ2 were detected by real-time quantitative PCR. When 70%-80% cells were confluent, the mRNA expressions of Col1a1, Scx, Tnmd and Dcn were also detected by real-time quantitative PCR.

RESULTS AND CONCLUSION: At the third passage, slender spindle-shaped cells were seen in both two groups, but there was a little change in the cell morphology in the chronic tendinopathy group. Lipid droplets were formed after the cells were cultured in adipogenic induction medium for 21 days. This was not observed in the control group. We observed more oil red O-positive oil droplets in tendon-derived stem cells from the tendons of chronic tendinopathy rats than healthy rats. The difference between them was statistically significant (P=0.004). The results of real-time quantitative PCR showed that the mRNA expressions of C/EBPα and PPARγ2 in the tendon-derived stem cells from the tendons of chronic tendinopathy rats were significantly higher than those in tendon-derived stem cells from the tendons of healthy rats (P=0.004); the mRNA expressions of Col1a1, Scx, Tnmd and Dcn in the tendon-derived stem cells from the tendons of chronic tendinopathy rats were significantly lower than those in tendon-derived stem cells from the tendons of healthy rats (P=0.009). In conclusion, tendon-derived stem cells from chronic tendinopathy rats showed a higher ability of adipogenic differentiation, but a lower capacity of tenogenic differentiation compared to tendon-derived stem cells from healthy rats, which might contribute to better understand the pathogenesis of tendinopathy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

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Key words: tissue engineering, stem cells, tendinopathy, rats

CLC Number:

R394.2

Chen Hui, Lin Yu-cheng, Xu Hong-liang, Wang Chen, Rui Yun-feng. Adipogenic and tenogenic differentiation of tendon-derived stem cells isolated from an animal model of chronic tendinopathy in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7320-7326.

Figures/Tables 5

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