Culture and identification of SK-N-SH neuroblastoma stem cells

doi:10.3969/j.issn.2095-4344.2014.45.009

Abstract

Abstract:

BACKGROUND: Neuroblastoma is a common solid tumor in children. Pediatric tumors are little affected by environmental factors, but closely related to child development. The suspension method is an effective and reliable method to harvest neoplastic stem cells.

OBJECTIVE: To culture the cell clones of human neuroblastoma cell line SK-N-SH and to assess the biological properties of the cell clones.

METHODS: Using the suspension method with no serum media, tumor cell clones were obtained. Immunofluorescence method was used to identify whether tumor cell clones exhibit stem cell properties. SK-N-SH neuroblastoma was labeled by luciferase, and tumor cell clones and tumor cells were seeded onto the back of nude mice to monitor cell proliferative properties in nude mice using in vivo imaging.

RESULTS AND CONCLUSION: Using the suspension culture method, SK-N-SH neuroblastoma cells could successfully develop into cloning balls. Under serum-free culture, cloning balls were immunofluorescently used to detect molecular markers that showed strong positive expression. Cloning balls subcutaneously implanted into nude mice showed the strong ability of self-renewal and differentiation as stem cells. The cell clones cultured by the suspension method strongly expressed Nestin, but weakly expressed glial fibrillary acidic protein, neuron-specific tubulin, possessing stem cell characteristics and strong proliferation and metastasis in nude mice.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: neoplasms, neuroblastoma, neoplastic stem cells, neural stem cells

CLC Number:

R394.2

Xie Yan, Su Yu-xi, Qin Jia-qiang, Nan Guo-xin, Huang Shi-feng, Wang Zhong-liang, Cai Wen-quan. Culture and identification of SK-N-SH neuroblastoma stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7260-7265.

Figures/Tables 5

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