Role of methylation of p21 gene in the proliferation of human vascular smooth muscle cells

doi:10.3969/j.issn.2095-4344.2014.38.002

Abstract

Abstract:

BACKGROUND: Proliferation, migration and phenotypic changes of vascular smooth muscle cells is the core of the occurrence of atherosclerosis, and a series of related genes via methylation are involved in the process.
OBJECTIVE: To investigate the effects of oxidized low density lipoprotein (ox-LDL) on DNA methylation in the promoter region of the p21 gene and its potential mechanism in the pathogenesis of atherosclerosis.
METHODS: Cultured human vascular smooth muscle cells were treated with different concentrations of ox-LDL (0, 10, 20, 40 mg/L) for 24 hours. The degree of DNA methylation was assayed by methylation-specific polymerase chain reaction, the expression of p21 mRNA was measured by reverse transcriptional polymerase chain reaction and the proliferative activity of vascular smooth muscle cells was determined by the MTT assay.
RESULTS AND CONCLUSION: The ox-LDL treatment resulted in a promotion in the methylation in the promoter region of the p21 gene and a decrease in mRNA expression with a concentration-dependent manner; it also induced a dose-dependent promoting effect on vascular smooth muscle cell proliferation. The atherogenic mechanism of ox-LDL might promote vascular smooth muscle cell proliferation by the hypermethylation of the p21 gene that may lead to the occurrence and development of atherosclerosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: genes, methylation, atherosclerosis, cyclins

CLC Number:

R318

Hou Xiao-fei, Sun Xiao-jian. Role of methylation of p21 gene in the proliferation of human vascular smooth muscle cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(38): 6074-6078.

Figures/Tables 1

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