Sustained-release capacity of bone morphogenetic protein composite scaffolds

doi:10.3969/j.issn.2095-4344.2014.21.011

Abstract

Abstract:

BACKGROUND: Clinical trials have proved that nano-hydroxyapatite/collagen bone scaffold has good tissue compatibility, suitable porosity and degradation performance, but it is limited by the lack of sustained-release growth factors and induced osteogenic capacity.
OBJECTIVE: To study the effect of the sustained-release system of polyethylene pyrrolinone modified nano-hydroxyapatite composite scaffold carrying bone morphogenetic protein.
METHODS: Samples were divided into three groups, and there were two cryogenic vials in each group. In the experimental group, a piece of 5 mm×5 mm×5 mm nano-hydroxyapatite/collagen bone was placed per vial and mixed with polyethylene pyrrolinone at -4 ℃ overnight, and bone morphogenetic protein solution was then added followed by vacuum pumping and cryopreservation. In the control group 1, a piece of 5 mm×5 mm×5 mm nano-hydroxyapatite/collagen bone was placed per vial and mixed with bone morphogenetic protein solution followed by vacuum pumping and cryopreservation. In the control group 2, a piece of 5 mm×5 mm×5 mm non-decalcified rat cancellous bone was placed per vial and mixed with polyethylene pyrrolinone at -4 ℃ overnight, and bone morphogenetic protein solution was then added followed by vacuum pumping and cryopreservation. After 14 days, the in vitro release activity of bone morphogenetic protein in the three groups was detected using enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: After 14 days, the absorbance values of bone morphogenetic protein in the two control groups were close to 0, whereas the experimental group showed a higher absorbance value (P < 0.05). It indicates that polyethylene pyrrolinone modified bone morphogenetic protein/nano-hydroxyapatite composite scaffold presents good sustained-release effects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, bone morphogenetic proteins, brace, delayed-action preparations

CLC Number:

R318

Xie Jiang-ning, Chen Jing-jia. Sustained-release capacity of bone morphogenetic protein composite scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3341-3344.

Figures/Tables 1

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