Systematic establishment for directed differentiation of induced pluripotent stem cells into hematopoietic progenitor cells in vitro

doi:10.3969/j.issn.2095-4344.2014.19.009

Abstract

Abstract:

BACKGROUND: There have been a large number of reports on establishing induced pluripotent stem cell lines, but studies concerning large-scale in vitro induced differentiation of induced pluripotent stem cells into hematopoietic progenitor cells still have a lack of in-depth discussion.
OBJECTIVE: To develop methods to induce differentiation of induced pluripotent stem cells into hematopoietic progenitor cells in vitro.
METHODS: Using the method of infection with lentivirus particles containing four transcriptionfactor genes, which are Oct4, Sox2, Nanog and Lin28, human skin fibroblasts are transduced into induced pluripotent stem cells. In the induced differentiation system, Y-27632, a kind of tyrosine kinase inhibitor-ROCK (p160-Rho-associated coiled-coil kinase), was added, which obviously suppressed apoptosis of cells. Based on conditioned medium with OP9 cells, a differentiation system of inducing induced pluripotent stem cells differentiating into hematopoietic progenitor cells was established.
RESULTS AND CONCLUSION: (1) Apoptosis of induced pluripotent stem cells at the first three passages was very obvious, and the cells were difficult in a large-scale expansion. After Y-27632 was added, the apoptosis of embryonic stem cells was obviously inhibited. (2) During embryoid body differentiation, induced pluripotent stem cells cultured in OP9 conditional growth medium differentiated into hematopoietic progenitor cells in vitro that were positive for CD34.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: induced pluripotent stem cells, embryoid bodies, hematopoietic stem cells, cell differentiation

CLC Number:

R394.2

Shen Jie, Li Jin-hui, Ruan Jing, Qiu Yun, Zhu Yin, Cui Da-xiang, Fan Zhi-hong, Wang Zheng . Systematic establishment for directed differentiation of induced pluripotent stem cells into hematopoietic progenitor cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(19): 3005-3011.

Figures/Tables 5

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