Human synovial fluid promotes directed differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.10.003

Abstract

Abstract:

BACKGROUND: Nowadays, growth factors are commonly used to induce bone marrow mesenchymal stem cells. However, this is a high-cost method with a great amount of growth factors. In addition, the chondrogenic potential of bone marrow mesenchymal stem cells will decrease significantly with increasing times of culture.
OBJECTIVE: To observe the directed differentiation of bone marrow mesenchymal stem cells co-cultured with human synovial fluid.
METHODS: Human bone marrow mesenchymal stem cells were isolated and cultured by adherence screening method. The synovial fluid of the knee was aspirated from healthy volunteers by aseptic operation. Passage 3 human bone marrow mesenchymal stem cells were co-cultured with the following media: synovial fluid+complete medium; synovial fluid+bone marrow mesenchymal stem cells+complete medium; bone marrow mesenchymal stem cells+complete medium. The morphology and growth of the cells were observed under an inverted microscope every day. At days 7, 14 and 21 of induction, toluidine blue staining and immunocytochemical staining were performed.
RESULTS AND CONCLUSION: After co-culture with human synovial fluid, human bone marrow mesenchymal stem cells proliferated slowly, and varied from fusiform to oval or polygonal; toluidine blue and collagen II staining were positive. These findings indicate that the synovial fluid has a positive role in the chondrogenic differentiation of bone marrow mesenchymal stem cells. The synovial fluid may contain substances that promote the chondrogenic differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: mesenchymal stem cells, synovial fluid, chondrocytes, cell culture techniques, collagen type II

CLC Number:

R394.2

Hua Qiang, Wu Jia-qi, Zhong Chuan-shan, Liu Zong-chao, Yan Guang-jian, Xiong Xiao-tian, Cui Xiao-ming . Human synovial fluid promotes directed differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1490-1495.

Figures/Tables 4

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