Effect of Zuoguiwan and Youguiwan on transforming growth factor beta1 and its signal transduction protein Smad2/3 in osteogenic induction of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.10.004

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can differentiate into osteoblasts under inducing condition that Zouguiwan and Youguiwan coordinate inducers, but the mechanism remains to be discussed.
OBJECTIVE: To observe the effects of serum containing Zuoguiwan and Youguiwan on transforming growth factor β1 and its signal transduction protein Smad2/3 message expression during the osteogenic differentiation of bone marrow mesenchymal stem cells.
METHODS: A whole bone marrow adherence method was adopted to isolate and cultivate bone marrow mesenchymal stem cells from rats. The cell cultivation was processed in five groups: bone marrow mesenchymal stem cells were respectively cultured with blank serum, serum containing Zouguiwan, serum containing Youguiwan, positive serum containing progynova+inducer (dexamethasone, vitamin C, and β-glycerophosphate), and inducer. Western blot was applied to detect the expression of type I collagen. The immunohistochemical assay was utilized to test transforming growth factor β1 and Smad2/3 expression in the osteoblasts.
RESULTS AND CONCLUSION: It was apparently more significant for serum containing Zuoguiwan and Youguiwan on type I collagen, transforming growth factor β1 and Smad2/3 expression, compared with blank serum group and inducer group (P < 0.05); moreover, serum containing Zuoguiwan was better than serum containing Youguiwan (P < 0.05). Both of serum containing Zuoguiwan and Youguiwan are able to promote osteogenic differentiation of bone marrow mesenchymal stem cells. Moreover, Zuoguiwan is much more effective indicating that this method of traditional Chinese medicine about nourishing kidneys can be better to promote osteogenic induction of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, bone marrow, osteoblasts, adaptor proteins, signal transducing, transforming growth factors

CLC Number:

R394.2

Sun Yue-jiao, Song Nan, He Wen-zhi, He Li-juan, Ren Yan-ling. Effect of Zuoguiwan and Youguiwan on transforming growth factor beta1 and its signal transduction protein Smad2/3 in osteogenic induction of rat bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1496-1501.

Figures/Tables 3

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