Biological characteristics and superiority of rat bone marrow mesenchymal stem cells isolated and cultured using whole bone marrow adherence method

doi:10.3969/j.issn.2095-4344.2014.10.002

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells are rare in vivo. It is important to purify, proliferate and differentiate bone marrow mesenchymal stem cells in vitro for further research.
OBJECTIVE: To evaluate the biological characteristics, phenotype and multiple differentiation potential cultivation of bone marrow mesenchymal stem cells that are isolated, cultured and purified using the whole bone marrow adherence method.
METHODS: Bone marrow mesenchymal stem cells were isolated, purified and cultured by the whole bone marrow adherence method. Morphological observation and flow cytometry determination of cell surface markers were performed. Osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells was induced.
RESULTS AND CONCLUSION: We successfully purified and proliferated bone marrow mesenchymal stem cells with high cell viability and differentiation ability. Fibroblast-like cells were harvested, expressing CD29 and CD90, but not CD45. Following osteogenic and adipogenic induction, cells were positive for oil red O staining and alizarin red staining. The whole bone marrow adherence method is easy to operate, has little impact on cell viability, and can be used to harvest high-purification bone marrow mesenchymal stem cells with high cell viability and differentiation ability.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, cells, cultured

CLC Number:

R394.2

Li Shuang-yue, Qi Yuan, Chen Ruo-lin, Wang Zhe-min, Liu Shuang, Piao Feng-yuan. Biological characteristics and superiority of rat bone marrow mesenchymal stem cells isolated and cultured using whole bone marrow adherence method[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1484-1489.

Figures/Tables 3

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