Establishment of diabetic cystopathy guinea pig model and its urodynamic evaluation

doi:10.3969/j.issn.2095-4344.2014.07.014

Abstract

Abstract:

BACKGROUND: Diabetic cystopathy is one of the most common chronic diabetic complications. The establishment of animal models of diabetic cystopathy will provide experimental animal platform for relevant research.
OBJECTIVE: To establish a guinea pig model of diabetic cystopathy and to evaluate its urodynamic characteristics.
METHODS: Fifty short-hair Britain female guinea pigs were randomly divided into two groups, 42 as the experiment group and the other 8 as the control group. The experiment group was intraperitoneally injected with streptozotocin to induce diabetes. The control group received injection of blank citric acid buffered solution. Diabetic guinea pigs were detected by urinary dynamics test at 9 and 12 weeks. Diabetic guinea pigs were further assigned into diabetic cystopathy subgroup and compensated subgroup. The urodynamic parameters of three groups were compared.
RESULTS AND CONCLUSION: Twenty of 42 guinea pigs were successfully induced diabetes by the injection of streptozotocin. At 9 weeks after the injection, bladder function compensation was present in six diabetic guinea pigs while bladder function was decompensated in another three diabetic guinea pigs. At 12 weeks, bladder function compensation was present in one diabetic guinea pig, while another eight guinea pigs were confirmed with diabetic cystopathy (88.89%). In the diabetic cystopathy subgroup, the residual urine volume was increased (0.72±0.08) mL, maximal detrusor pressure was decreased (0.63±0.05) kPa, maximum bladder capacity was increased (2.01±0.05) mL, and bladder compliance was increased (0.34±0.04) mL/kPa. There were significant differences compared with the compensated subgroup and the control group (P < 0.001). Diabetic cystopathy occurs at 12 weeks after diabetic models are successfully established in guinea pigs, and urodynamic changes are mainly the increase of residual urine volume.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: diabetes mellitus, urinary bladder diseases, guinea pigs, models, animal, blood glucose, urination

CLC Number:

R318

Luo Guang-cheng, He Zhi-hua, Luo Jian-zhen, Xu Yi-ming, Ma Hong . Establishment of diabetic cystopathy guinea pig model and its urodynamic evaluation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1063-1068.

Figures/Tables 2

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