Berberine alleviates ischemia/reperfusion injury in type 2
diabetic rats

doi:10.3969/j.issn.2095-4344.1904

Abstract

Abstract:

BACKGROUND: Berberine has been shown to alleviate cerebral ischemia/reperfusion injury.

OBJECTIVE: To investigate the effects of berberine on the cerebral ischemia/reperfusion injury in type 2 diabetic rats and its molecular mechanism.

METHODS: The study was approved by the Ethical Committee of Chinese Medicine Hospital of Hainan Province. The rats underwent intraperitoneal injection of 30 mg/kg streptozotocin, twice every other day, for 8 weeks to establish the type 2 diabetic model. Ninety male Sprague-Dawley rats with average blood sugar ≥ 11.1 mmol/L were selected and randomly divided into control, ischemia/reperfusion injury and ischemia/reperfusion injury+berberine groups (n=30/group). The rats in the former two groups were given the normal saline via gavage, and the ischemia/reperfusion injury+berberine group received the 200 mg/(kg·d) via gavage. After 7 days of treatment, the latter two group rats were subjected to middle cerebral artery occlusion for 2 hours and reperfusion for 12 hours to establish the model of middle cerebral artery ischemia/reperfusion. Hematoxylin-eosin staining and transmission electron microscopy were used to observe the volume of cerebral infarction. The levels of superoxide dismutase, malondialdehyde and nitric oxide in the infarct area were detected by ELISA. The brain cell apoptosis was detected by TUNEL staining. The expression levels of PI3K, Akt and phosphorylated Akt were detected by western blot assay.

RESULTS AND CONCLUSION: (1) Compared with the ischemia/reperfusion group, the volume of cerebral infarction in the ischemia/ reperfusion+berberine group was significantly reduced (P < 0.05), the superoxide dismutase level was significantly increased, and the malondialdehyde and nitric oxide levels were significantly decreased. (2) Compared with the ischemia/reperfusion group, the ischemia/ reperfusion+berberine group had less apoptosis, higher expression of Bcl-2 and lower expression of cleaved Caspase-3 and Bax. (3) Compared with the ischemia/reperfusion group, the ischemia/reperfusion+berberine group had increase in the expression levels of PI3K and p-Akt. (4) These results indicate that berberine can play an anti-apoptotic role by activating PI3K-Akt signaling pathway and alleviate brain ischemia/ reperfusion injury in type 2 diabetic rats.

Key words: ischemia/reperfusion, type 2 diabetes mellitus, berberine, apoptosis, PI3K-Akt

CLC Number:

R446

R496

R318

Fu Yunyu, Qiu Xiaotang, Yang Wenkui, Mo Shian, Wu Xiaocui, Wu Yingping. Berberine alleviates ischemia/reperfusion injury in type 2 diabetic rats[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(2): 230-235.

Figures/Tables 5

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