Molecular network of miR-106b-5p regulating osteoporotic fracture in the elderly

doi:10.12307/2022.039

Abstract

Abstract: BACKGROUND: Osteoporotic fractures are age-related diseases, with increasing incidence and prevalence due to the aging population.
OBJECTIVE: To identify whether miRNA is associated with osteoporotic fracture.
METHODS: The differential expression of miRNA (DEM) related to osteoporotic fracture in GSE93883 was analyzed by limma package. The target genes of differentially expressed miRNA were predicted by RAID database, and the key miRNAs and target genes were identified by protein-protein interaction network. Enrichment analysis identified the biological functions and signaling pathways involved in target genes. Finally, qPCR and western blot were used to verify the key miRNAs and signal pathways in bone tissue of five elderly patients with osteoporotic fractures and five elderly patients with non-osteoporotic fractures.
RESULTS AND CONCLUSION: A total of 21 differentially expressed miRNAs related to osteoporotic fracture were obtained and 530 target genes were predicted. MiR-106b-5p was identified as a key regulatory gene by protein-protein interaction network. The target genes were mainly related to the activation of MAPK activity, PI3K-Akt signaling pathway and transforming growth factor β signaling pathway. In addition, qPCR results confirmed significant downregulation of miR-106b-5p in elderly osteoporotic fracture (P < 0.05). Western blot results showed that PI3K-Akt and transforming growth factor β signaling pathways were significantly activated in elderly osteoporotic fractures. Therefore, miRNA is involved in elderly osteoporotic fracture and is associated with PI3K-Akt and transforming growth factor βsignaling pathways. MiR-106b-5p may inhibit the occurrence of osteoporotic fractures and become a potential marker and therapeutic target.

Key words: osteoporotic fracture, miR-106b-5p, PI3K-Akt, transforming growth factor, differential expression

CLC Number:

Wang Hailong, Li Long, Yilihamu Tuoheti, Liu Xu, Nacikedaoerji, Chen Hongtao, Cui Yong. Molecular network of miR-106b-5p regulating osteoporotic fracture in the elderly[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(2): 239-244.

Figures/Tables 4

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