Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (34): 5445-5452.doi: 10.12307/2021.237
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Yang Zhen1, 2, Li Hao1, 2, Fu Liwei1, 2, Gao Cangjian1, 2, Jiang Shuangpeng2, Wang Fuxin2, Yuan Zhiguo2, Sun Zhiqiang1, 2, Zha Kangkang1, 2, Tian Guangzhao1, 2, Cao Fuyang2, Sui Xiang2, Liu Shuyun2, Guo Quanyi2
Received:
2020-06-28
Revised:
2020-07-03
Accepted:
2020-08-04
Online:
2021-12-08
Published:
2021-07-26
Contact:
Guo Quanyi, Professor, Institute of Orthopedics, the First Medical Center, Chinese PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Beijing 100853, China
About author:
Yang Zhen, Master candidate, Medical College of Nankai University, Tianjin 300071, China; Institute of Orthopedics, the First Medical Center, Chinese PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries, PLA, Beijing 100853, China
Supported by:
CLC Number:
Yang Zhen, Li Hao, Fu Liwei, Gao Cangjian, Jiang Shuangpeng, Wang Fuxin, Yuan Zhiguo, Sun Zhiqiang, Zha Kangkang Tian Guangzhao, Cao Fuyang, Sui Xiang, Liu Shuyun, Guo Quanyi. Cartilage composite scaffold loaded with transforming growth factor beta 3 using three-dimensional bioprinting[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(34): 5445-5452.
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2.4 3D打印支架生物力学检测 3D生物打印PCL/GelMA/ECM支架的压缩模量为(14.24±2.44) MPa,高于PCL支架的(10.41±1.13) MPa(P < 0.05)和人膝关节正常软骨压缩模量(4.3-13.0 MPa)[28],具有良好的生物力学性能,可以在软骨再生的时间窗口内提供力学支撑。 2.5 3D打印支架缓释性检测 负载于GelMA/ECM生物墨水中的TGF-β3累计释放曲线见图4。在缓释检测的60 d内,前1周内释放较快,因子累计释放率已达约60%,呈现短期内的突释现象,随后的1-4周释放速度逐渐减缓,而4-8周的释放规律类似于零级释放动力学,60 d累计释放率约达80%,并且仍有缓慢释放的趋势。"
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