Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (5): 724-729.doi: 10.3969/j.issn.2095-4344.2014.05.012

Previous Articles     Next Articles

Serum protein fingerprint pattern model for diagnosing blood stasis syndrome of lumbar intervertebral disc protrusion

Xu Jian-wen1, Wei Gui-kang2, Zhong Yuan-ming1, Yin Li-jun1, Zhou Bin-bin1, Wei Yu-lan2, Li Zhi-fei1, Song Quan-sheng1, Hu Bing3, Ji Jing4   

  1. 1Department of Orthopedics, First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China; 2Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 3Grade 2009 candidate for the Master’s degree, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 4Grade 2008 candidate for the Master’s degree, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China
  • Revised:2013-12-31 Online:2014-01-29 Published:2014-01-29
  • About author:Xu Jian-wen, M.D., Professor, Chief physician, Department of Orthopedics, First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81060300; Natural Science Foundation of Guangxi Zhuang Autonomous Region in China, No. 2011GXNSFA018181; Excellent Talent Project for Guangxi Universities, No. 201092

PDF

493

Abstract:

BACKGROUND: The correlation between blood stasis syndrome and non-blood stasis syndrome of lumbar intervertebral disc protrusion remains unclear.

OBJECTIVE: To construct serum protein pattern model for diagnosing blood stasis syndrome of lumbar intervertebral disc protrusion.
METHODS: A total of 180 cases were included in this study and divided into treatment group (120 patients with lumbar intervertebral disc protrusion) and control group (60 healthy cases from physical examination). Furthermore treatment group was equally assigned into blood stasis syndrome subgroup and non-blood stasis syndrome subgroup, with 60 cases in each subgroup. The involved cases were well matched in nations, genders and ages. Serum samples of peripheral blood from the 180 cases were collected. Surface-enhanced laser desorption/inionation time of flight mass spectrometry and ProteinChip technology were employed to detect and plot protein mass spectrum. The protein peak values were identified using Biomarker Wizard software. Then serum diagnosis model of blood stasis syndrome of lumbar intervertebral disc protrusion was established. The obtained models were verified through double blind method. The differential proteins were searched by ExPASy data.

RESULTS AND CONCLUSION: We detected that peak values of eleven proteins had statistical significance (P < 0.05) from the involved 180 cases. Among them, two proteins were highly expressed while the other nine proteins were lowly expressed. Serum protein pattern model for diagnosing blood stasis syndrome of lumbar intervertebral disc protrusion was established through Biomarker Patterns software, and the sensibility was 86.667%, the specificity was 94.167%, the positive predictive value was 88.136%. There are a variety of abnormally expressed proteins in the serum of the patients with blood stasis syndrome of lumbar intervertebral disc protrusion. The serum protein pattern model involved eleven different proteins can be used to diagnose blood stasis syndrome of lumbar intervertebral disc protrusion.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: intervertebral disk displacement, proteomics, proteinchip array analysis

CLC Number: