Differential proteomics study of patients with sternal ossification of the ligamentum flavum

doi:10.3969/j.issn.2095-4344.3202

Abstract

Abstract: BACKGROUND: The research on the pathogenesis and mechanism of thoracic ossification of the ligamentum flavum mainly focuses on the research of functional genome; however, less is reported on it from proteomics.
OBJECTIVE: To investigate the differential proteins between ossified ligamentum flavum and normal ligamentum flavum in patients with thoracic ossification of the ligamentum flavum, and to search for disease-related target proteins.
METHODS: Ossified ligamentum flavum and adjacent normal ligamentum flavum samples were collected from three patients with thoracic ossification of the ligamentum flavum, and detected through protein extraction, quantification, isotope labeling for relative and absolute quantitative technique-labeled peptides. Differentially expressed proteins were identified and screened by liquid chromatography-tandem mass spectrometry.
RESULTS AND CONCLUSION: Totally 589 kinds of differentially expressed proteins were identified, including 532 up-regulated proteins and 57 down-regulated proteins. Further analysis identified 23 kinds of proteins related to inflammation, among which, the expression of CYBB, PODXL, S100A12, LXN, S100A10, GJA1, LIPA, FOLR2, CYBA, IFI16, ABCF1, HMGB1, RPS19, PRDX5, MAP2K3 was up-regulated, and the expression of KRT14, IL17D, IL1RAP, SLC7A2, RHOB, COMP, KRT6B was down-regulated. These inflammation-related proteins may be important diagnostic and therapeutic targets involved in the occurrence and development of thoracic ossification of the ligamentum flavum.

Key words: ligamentum flavum, ossification, proteomics, tissue, TMT technology

CLC Number:

Hu Wandong, He Jialin, Zhang Longsheng, Liao Wenbo. Differential proteomics study of patients with sternal ossification of the ligamentum flavum[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(17): 2625-2629.

Figures/Tables 3

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