Non-surgical treatment of lumbar intervertebral disc protrusion without blood stasis: a serum proteomic analysis

doi:10.3969/j.issn.2095-4344.0835

Abstract

Abstract:

BACKGROUND: Proteomics is a well studied research method, but its application in the non-surgical treatment of lumbar intervertebral disc protrusion (LIDP) is little reported.
OBJECTIVE: To screen the differentially expressed proteins in patients with LIDP but without blood stasis before and after non-surgical treatment by proteomics.
METHODS: Sixty patients with LIDP but without blood stasis were selected, and treated with non-surgical treatment for 4 weeks. The differentially expressed proteins were screened and identified by iTRAQ combined with LC-MS/MS. The bioinformatics analysis of the identified proteins was carried out, and the curative effectiveness was investigated.
RESULTS AND CONCLUSION: Compared with those before treatment, the Visual Analogue Scale scores were significantly (P < 0.05), the Japanese Orthopedic Association scores were significantly increased decreased (P < 0.05), and the excellent and good rate reached 95.0% post-treatment. A total of 300 differentially expressed proteins were screened and 25 significantly expressed proteins were identified (P < 0.05). Bioinformatics analysis revealed that nine of the significantly expressed proteins were enriched to 15 KEGG signaling pathways. These results suggest that the use of Western medicine non-surgical treatment for the LIDP without blood stasis can achieve satisfactory results. Besides, complement C1qA, cDNA protein (FLJ60724), complement C4B frameshift mutation, cDNA protein (FLJ53025), mannose binding protein C, apolipoprotein B, hemoglobin α-1 globin chain variant, hemoglobin β subunit and cDNA protein (FLJ76254) may be the potential serum markers of the non-surgical treatment for the LIDP without non-blood stasis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Intervertebral Disc Displacement, Isotope Labeling, Proteomics, Tissue Engineering

CLC Number:

R318

Gui Yu-chang1, Xu Jian-wen2, Tai Zhi-hong1, Rao Yuan-sen1, Cao Yu-ju1, Yin Li-jun3. Non-surgical treatment of lumbar intervertebral disc protrusion without blood stasis: a serum proteomic analysis[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(16): 2570-2576.

Figures/Tables 3

2.3 质谱鉴定结果通过iTRAQ标记技术联合LC-MS/ MS技术共鉴定到300个蛋白质组，筛选出治疗前后表达差异的蛋白质25个，其中上调表达的差异蛋白11个、下调表达的差异蛋白14个(见表2)。 2.4 差异蛋白GO功能注释聚类分析生物过程方面发现差异蛋白主要参与了单一生物过程、细胞过程、应激反应、生物调节、代谢过程、免疫系统过程、多细胞过程、细胞定位、信号传导、细胞成分组织、发育过程、多生物过程、生长过程、细胞移动和生物黏附(具体注释见图1A)。分子功能方面发现差异蛋白主要涉及了结合、催化活性、转运活性、酶调节活性、抗氧化活性、分子传导活性、结构分子活性、受体活性(具体注释见图1B)。细胞构成方面这些差异蛋白主要定位在细胞器、胞外区、细胞、细胞膜、大分子复合物组分、细胞外基质和膜蛋白等部位(具体注释见图1C)。 2.5 差异蛋白KEGG通路注释聚类分析应用KASS (KEGG Automatic Annotation Server)通过同源/相似蛋白的KO号注释至相关KEGG通路，进一步分析22个差异蛋白，结果提取到被注释至15条KEGG信号/代谢通路的9个差异蛋白：补体C1qA、cDNA(FLJ60724)蛋白、补体C4B移码突变、cDNA(FLJ53025)蛋白、甘露糖结合蛋白C、载脂蛋白B、血红蛋白α-1珠蛋白链变体、血红蛋白β亚基和cDNA(FLJ76254)蛋白。15条KEGG信号/代谢通路中补体与凝血级联、金黄色葡萄球菌感染富集到4个差异蛋白，百日咳、系统性红斑狼疮、疟疾富集到3个差异蛋白，细胞吞噬体、非洲锥虫病富集到2个差异蛋白，PI3K-Akt信号通路、粘着斑、ECM受体作用、叶酸合成、朊粒病、美洲锥虫病、脂肪消化吸收、维生素消化吸收8条信号通路富集到1个差异蛋白(见表3)。"

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