Hydrolysis of Nanzhu fluid promotes proliferation and migration of human microvascular endothelial cells

doi:10.3969/j.issn.2095-4344.0215

Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (16): 2564-2569.doi: 10.3969/j.issn.2095-4344.0215

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Hydrolysis of Nanzhu fluid promotes proliferation and migration of human microvascular endothelial cells

Cen Yan-hui1, 2, Lin Yong3, Lin Jiang1, Jia Wei1, Zhao Jing1

1Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 2the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China; 3Beihai Baozhulin Marine Technology Co., Ltd., Beihai 536000, Guangxi Zhuang Autonomous Region, China

Received:2018-03-01 Online:2018-06-08 Published:2018-06-08
Contact: Lin Jiang, Doctoral candidate, Professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China Corresponding author: Jia Wei, Master, Associate professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China
About author:Cen Yan-hui, M.D., Associate professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China Lin Yong, Senior engineer, Beihai Baozhulin Marine Technology Co., Ltd., Beihai 536000, Guangxi Zhuang Autonomous Region, China Cen Yan-hui and Lin Yong contributed equally to this work.
Supported by:
the National Industrial Chain Coordination Innovation Project of Marine Economy Innovation and Development Model City (Beihai City), No. Bhsfs002; the Key Project of Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2015GXNSFDA227002; the Science and Technology Base and Talent Specialization of Guangxi Zhuang Autonomous Region, No. AD17195080; Guangxi Specialist Posts of “Beibu Gulf Marine Medicine Research and Development”; the National Postdoctoral Science Foundation of China, No. 2017M623297XB; Qihuang Engineering High-Level Talent Team Cultivation Project of Guangxi University of Chinese Medicine, No. 2018001

Abstract

Abstract:

BACKGROUND: Pearl has the tranquilizing effect, and it can be applied in the treatment of hypertension. However, there is little report on the prevention and cure effect and mechanism of hydrolyzed pearl liquid on hypertension.
OBJECTIVE: To observe the effect of hydrolysis of Hepu pearl (hydrolyzed Nanzhu fluid) on the biological behavior and secretion of human microvascular endothelial cells.
METHODS: Human microvascular endothelial cells were cultured and passaged. There were four groups, and the microvascular endothelial cells were incubated in the 200 μL culture medium containing nothing (control group), and 120, 60 and 30 mg/L hydrolysis of Nanzhu fluid. The cell proliferation and migration was detected by cell conuting kit-8 assay and Transwell assay respectively; the cell cycle distribution was tested by flow cytometry; the cell apoptosis was assayed by TUNEL method; the secretion of nitric oxide and reactive oxygen species was tested by nitrale reduetase and chemical fluorescence method, respectively.
RESULTS AND CONCLUSION: Compared with the control group, hydrolysis of Nanzhu fluid significantly promoted the proliferation of microvascular endothelial cells in a manner-dependent manner (P < 0.05), suggesting the optimal concentration was 120 mg/L. Compared with the control group, the percentage of cells in S and G2 phase was significantly increased, and the percentage of cells in the G1 phase was significantly reduced in the hydrolysis of Nanzhu fluid group (P < 0.05), indicating hydrolysis of Nanzhu fluid could promote cell cycle progression. Apoptotic cells with green-stained nucleus were invisible in both groups. The number of cell migration in the hydrolysis of Nanzhu fluid group was significantly more than that in the control group (P < 0.05). Compared with the control group, there was a significant increase in the nitric oxide secretion, and a significant decrease in the production of reactive oxygen species in the hydrolysis of Nanzhu fluid group (P < 0.05). To conclude, hydrolysis of Nanzhu fluid can promote the proliferation and migration of human microvascular endothelial cells in vitro, and has the function of promoting the secretion of nitric oxide and inhibiting reactive oxygen species secretion, implying its positive role in the protection of endothelial cell function.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hypertension, Endothelial Cells, Cell Movement, Tissue Engineering

CLC Number:

R318

Cen Yan-hui1, 2, Lin Yong3, Lin Jiang1, Jia Wei1, Zhao Jing1. Hydrolysis of Nanzhu fluid promotes proliferation and migration of human microvascular endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(16): 2564-2569.

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Hydrolysis of Nanzhu fluid promotes proliferation and migration of human microvascular endothelial cells

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