Effects of exercise training on bradykinin expression in rats with myocardial infarction

doi:10.3969/j.issn.2095-4344.2014.02.013

Abstract

Abstract:

BACKGROUND: Exercise can elicit generation of coronary collateral circulation through numerous pro-angiogenic growth factors. It is not enough via a single factor to clearly definite signaling pathways for the generation of collateral circulation. Many pro-angiogenic growth factors are involved in kallikrein-kinin system, and there is no report about exercise effects on this system.
OBJECTIVE: To study effects of exercise training on bradykinin expression in rats with myocardial infarction.
METHODS: Thirty Wistar rats were randomly assigned to control group, myocardial infarction group and exercises training group. Rats in the control group were subjected to thoracotomy with no coronary artery ligation. Rats in the other two groups were modeled. In the exercise training group, 3 days after myocardial infarction, rats were subjected to 30-minute exercise training on treadmill, once a day for 4 weeks. At the terminal of the experiment, blood samples were obtained to analyze bradykinin expression by enzyme linked immunosorbent assay, and the myocardium was sampled to analyze relative blood flow by microsphere method.
RESULTS AND CONCLUSION: After exercise training, bradykinin level in the exercise training group was significantly higher than that in the myocardial infarction group (P < 0.001). At the end of experiment, the relative blood flows of the exercise training and myocardial infarction groups were both increased compared with before experiment (P < 0.05, P < 0.001). At the end of experiment, the relative blood flow of the exercise training group was significantly higher than that of the myocardial infarction group (P < 0.01). In all the three groups, bradykinin level was significantly correlated with the relative blood flow in the myocardium. These findings indicate exercises training can improve bradykinin expression apparently and increase blood flow in the myocardium, which illustrates that the kallikrein-kinin system plays an important role in exercise induced angiogenesis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: myocardial infarction, exercise therapy, bradykinin, enzyme-linked immunosorbent assay, kallikreins

CLC Number:

R318

Shen Mei, Yu Min, Che Shi-qin, Jin Ke-xin, Ma Li, Wang Ping. Effects of exercise training on bradykinin expression in rats with myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(2): 239-244.

Figures/Tables 2

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