Attachment and proliferation of bone marrow mesenchymal stem cells cultured on porous tantalum rod

doi:10.3969/j.issn.2095-4344.2013.42.005

Abstract

Abstract:

BACKGROUND: Porous tantalum rods, possessing high porosity and highly similar elastic modulus to the trabecular bone, not only can provide effective mechanical support for the femoral head, but also can enhance revascularization in the areas of necrosis, reduce stress shielding, and provide guarantee for bone ingrowth in necrotic area.
OBJECTIVE: To investigate the attachment and proliferation of bone marrow mesenchymal stem cells on the surface of porous tantalum rod.
METHODS: The purified bone marrow mesenchymal stem cells which were separated from canines were seeded on the surface of porous tantalum rod by the cell density of 1.5×109/L. Cell attachment and proliferation was observed under phase microscope and scanning electron microscope at 5, 10 and 15 days under co-cultured condition.
RESULTS AND CONCLUSION: (1) Inverted microscope: Within 1-5 days after co-culture, the cells began to proliferate, and less cells were found near the tantalum rod, but more cells far from the rod. At 6-10 days after co-culture, the cells increased significantly and gradually migrated to the rod, and even some cells attached to the edges of the rod. After 14 days, the cells were interconnected to form a film, packing the surrounding and dents of the tantalum rod. Cells were visible to agglomerate and cell calcification occurred. (2) Scanning electron microscope: At 5 days after co-culture, no cell adhesion appeared on the the tantalum rod surface. At 10 days after co-culture, the cells scattered on the surface of tantalum rod, but there was no interconnection between them. After 15 days, cells were polygon shaped and connected into pieces. It could be seen that the cells secreted large amounts of collagen fibers, and the cells that were fusiform and polygon shaped were surrounded by a great amount of extracellular matrice. Bone marrow mesenchymal stem cells appear to have good adhesion and proliferation capability on the surface of tantalum rod.

Key words: biomaterials, stem cells, femur head necrosis, cell proliferation

CLC Number:

R318

Zhao Zhen-hua, Zhao De-wei, Fu Wei-min, Wang Ben-jie, Wei Xiao-wei, Wang Wei, Liu Bao-yi. Attachment and proliferation of bone marrow mesenchymal stem cells cultured on porous tantalum rod[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(42): 7369-7374.

Figures/Tables 4

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