Granulocyte-colony stimulating factor improves motor function of rats with spinal cord injury

doi:10.3969/j.issn.2095-4344.2013.40.014

Abstract

Abstract:

BACKGROUND: Recently, a neuroprotective effect of granulocyte colony-stimulating factor was reported in a model of cerebral infarction and a model of acute spinal cord injury. However, the applied animal model was not established by impact method, different from pathophysiological process of human.
OBJECTIVE: To observe effects of granulocyte colony-stimulating factor on motor function in a rat model of spinal cord injury induced by Allen’s method.
METHODS: Wistar rats were used to establish spinal cord injury at T10 level using modified Allen’s method. They were randomly assigned to two groups, granulocyte colony-stimulating factor group, treated with granulocyte colony-stimulating factor, and vehicle group, treated with equal volume of PBS. The motor function was evaluated with Basso-Beattie-Bresnahan score and modified Rivlin loxotic plate test monitored at 1, 7, 14, 21, 28 and 35 days, and four-limb muscle strength was assessed using Grid walk test at 7, 14, 21, 28 and 35 days post-operatively.
RESULTS AND CONCLUSION: Hind limbs paralysis occurred in all animals postoperatively. Scores of Basso-Beattie-Bresnahan and modified Rivlin loxotic plate test were greater in granulocyte colony-stimulating factor group compared with vehicle group at 7, 14, 21, 28 and 35 days (P < 0.05-0.01); mean Grid walk test errors were less in granulocyte colony-stimulating factor group compared with vehicle group at 14, 21, 28 and 35 days (P < 0.05-0.01). Results indicate that motor function and four-limb muscle strength were improved following granulocyte colony-stimulating factor therapy compared with vehicle group, indicating that granulocyte
colony-stimulating factor has a positive effect on spinal cord injury.

Key words: stem cells, spinal cord injuries, granulocyte colony-stimulating factor, muscle strength, models, animal

CLC Number:

R394.2

Li Zhao-cheng, Wang Wen-ji, Zhang Jing-gui, Zhao Long, Zhao Hui. Granulocyte-colony stimulating factor improves motor function of rats with spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(40): 7110-7116.

Figures/Tables 6

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