Shape-memory polymer based on D,L-poly(lactic acid): Biosecurity and cytocompatibility

doi:10.3969/j.issn.2095-4344.2013.38.008

Abstract

Abstract:

BACKGROUND: Previous experiments have prepared shape memory polymer based on D,L-poly(lactic acid). According to domestic technical requirements for biologically evaluating biomaterials and medical equipments, tissue-engineered grafts must be subjected to preclinical experiment for biosecurity and cytocompatibility evaluation.
OBJECTIVE: To observe the biosecurity of the shape memory polymer based on D,L-poly(lactic acid).
METHODS: (1) Bacterial endotoxin test: polymer extract, endotoxin working standard solution and checking standard water were added into limulus reagent, respectively. (2) Sensitization test: Polymer extract + Freund’s complete adjuvant + physiological saline and Freund’s complete adjuvant + physiological saline were injected into the scapula of Kunming mice. After induction by intradermal injection, local induction and excitation, stimulate skin erythema and edema degree were observed in animals. (3) Acute toxicity test: Kunming mice received intraperitoneal injection of 100%, 50%, 25% polymer leaching solution and physiological saline, respectively. (4) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for cell proliferation: The direct method was that human umbilical cord vascular endothelial cells were inoculated onto the polymer film, lactic acid and glass, respectively; the indirect method was that human umbilical cord vascular endothelial cells were inoculated into
the polymer leaching solution, acrylamide solution and 1640 culture solution.
RESULTS AND CONCLUSION: This shape memory polymer based on D,L-poly(lactic acid) is free of bacterial contamination in compliance with the biosecurity standards, and it has no allergenic and toxicity but has good cytocompatibility.

Key words: biocompatible materials, histocompatibility, polymers, acute toxicity tests

CLC Number:

R318

Xu Jian, Peng Kun, Gan Xiao-ling, Wang Yi-zhen, Duan Qiao-ling, Li Yong-gang. Shape-memory polymer based on D,L-poly(lactic acid): Biosecurity and cytocompatibility[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.38.008.

Figures/Tables 7

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