Serum-free culture promotes rat adipose-derived stem cells differentiating into endothelial cells

doi:10.3969/j.issn.2095-4344.2013.36.010

Abstract

Abstract:

BACKGROUND: There are few reports about the effect of serum-free culture on the differentiation of rat adipose-derived stem cells into vascular endothelial cells.
OBJECTIVE: To investigate the isolation, serum-free culture of rat adipose-derived stem cells differentiating into vascular endothelial cells.
METHODS: The rat adipose-derived stem cells were isolated from male Sprague-Dawley rats and expanded to the third passage by enzymatic digestion-adherent explants method. In the experimental group, rat adipose-derived stem cells were cultured in serum-free medium for 24 hours. In the control group, rat adipose-derived stem cells were cultured in low-glucose Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum. After that, the cells were cultured in inducing medium for 3 weeks to differentiate into vascular endothelial cells.
RESULTS AND CONCLUSION: The rat adipose-derived stem cells grew as polygonal or fusiform-shaped adherent cells when cultured in vitro, which could stably proliferate and passage. The rat adipose-derived stem cells showed very low expression of CD31, a cell surface marker, after passages. After directional differentiations into vascular endothelial cells, the cells were pebble-shaped under the inverted microscope. Expression of CD31 was up-regulated, which was much higher in the experimental group than the control group. The induced cells in the experimental group had stronger abilities than those in the control group to swallow Dil-labeled acetylated low-density lipoprotein and form tube-like structures on the matrigel after differentiation into vascular endothelial cells. So, rat adipose-derived stem cells could be highly successfully induced to differentiate into vascular endothelial cells in vitro after serum-free culture.

Key words: stem cells, adipocytes, endothelial growth factors, culture media, serum-free

CLC Number:

R394.2

Guo Huan-shan, Yan Ling. Serum-free culture promotes rat adipose-derived stem cells differentiating into endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(36): 6443-6448.

Figures/Tables 4

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