Reconstruction of quadriceps femoris by anatomizing the ventral roots of spinal nerves in rats with spinal cord injury

doi:10.3969/j.issn.2095-4344.2013.24.019

Abstract

Abstract:

BACKGROUND: Reconstruction of motor function of limbs in spinal cord injury is significant for patients with paraplegia.
OBJECTIVE: To recover the innervations and muscle contraction function of quadriceps femoris in spinal cord injured rats by constructing the neural pathway using the ventral roots of spinal nerves above injured
segment of spinal cord and lumbar nerve that dominates the quadriceps femoris.
METHODS: L₁ ventral root was anatomized to L₃ventral root from Sprague-Dawley rats. After 6 months, axonal regeneration was found from L₁ ventral root to L₃ ventral root through the grafting nerve, which may reconstruct muscle contraction function. After nerve suture for 6 months, spinal cord injury was made in L₂segment for neuroelectrophysiology examination to observe innervation of quadriceps femoris.
RESULTS AND CONCLUSION: After semi-dissection of L₂ segment, wave from quadriceps femoris was recorded in electromyogram meter when stimulating the grafting nerve. After injured the segment of L₂, we also record wave from quadriceps femoris when stimulating L₁ ventral root. Results showed that anastomosis of the L₁ ventral roots of spinal nerves above injured segment of spinal cord and the ventral root below the injured spinal cord segment can reconstruct the reflex path of innervation of quadriceps femoris, and the reflex center of quadriceps femoris in the spinal cord moves up to L₁ segment.

Key words: tissue construction, basic experiments in tissue construction, spinal nerve, spinal cord injury, nerve grafting, quadriceps femoris, muscle contraction, electromyogram, axon regeneration, reflex center, Sprague-Dawley rats, electrophysiology, National Natural Science Foundation of China

CLC Number:

R318

Li Wei, Zhong Gui-bin. Reconstruction of quadriceps femoris by anatomizing the ventral roots of spinal nerves in rats with spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4502-4508.

Figures/Tables 3

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