Biocompatibility of olfactory ensheathing cells and poly-L-lactic acid reinforced by chitosan in vitro

doi:10.3969/j.issn.2095-4344.2013.29.009

Abstract

Abstract:

BACKGROUND: Previous studies have shown that composite scaffold of chitosan and poly-L-lactic acid has good biocompatibility with some cells.
OBJECTIVE: To study the biocompatibility of poly-L-lactic acid reinforced by chitosan and olfactory ensheathing cells.
METHODS: In experimental group, olfactory ensheathing cells from Sprague-Dawley rats aged 1-3 days were incubated onto chitosan-reinforced poly-L-lactic acid film. And in control group, olfactory ensheathing cells were co-cultured with poly-L-lysine. The proliferative ability of olfactory ensheathing cells was detected and the cells were observed with immunofluorescence histochemical staining at 1, 3, 5, 7 days after culture.
RESULTS AND CONCLUSION: Olfactory ensheathing cells could survive on the chitosan-reinforced poly-L-lactic acid film, and the cytotoxic grade was Ⅰ. Morphology of the cells in the experimental group was round or oval, with little processes and the cells aggregated into groups. One day after implantation, the periphery cells of the mass extended short projections and gradually spread outward; 3 days after implantation, the cells spread and most of the cells generated projections, most of which were bipolar or tri-polar; 5 days after implantation, cell processes significantly extended, most cells were bipolar and tri-polar cells, while some were oval cells and irregular triangular cells; 7 days after implantation, the cell density increased, and cell processes extended. Cell morphology of the control group had similar characteristics as the experimental group. There was no obvious difference between the control and the experimental group in number, perimeter or area of the cells (P > 0.05). It showed that chitosan-reinforced poly-L-lactic acid had good biocompatibility with olfactory ensheathing cells.

Key words: biomaterials, material biocompatibility, chitosan, poly-L-lactic acid, poly-L-lysine, olfactory ensheathing cells, biocompatibility, co-culture, National Natural Science Foundation of China

CLC Number:

R318

Li Wei-dong, Cui Zhi-ming, Xu Guan-hua, Fan Jian-bo, Bao Guo-feng, Sun Yu-yu, Wang Ling-ling. Biocompatibility of olfactory ensheathing cells and poly-L-lactic acid reinforced by chitosan in vitro[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(29): 5316-5322.

Figures/Tables 5

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