Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (24): 4409-4413.doi: 10.3969/j.issn.2095-4344.2013.24.006

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Left ventricular papillary muscle ablation in canines by ultrasound ablation catheter

Wang Qi-feng1, Wang Zhi-gang2, Huang Jing1   

  1. 1 Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
    2 Ultrasonic Imaging Research Institute, Chongqing Medical University, Chongqing  400010, China
  • Received:2013-01-09 Revised:2013-02-21 Online:2013-06-11 Published:2013-06-11
  • Contact: Huang Jing, Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China huangjing_9901@yahoo.com.cn
  • About author:Wang Qi-feng★, Master, Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China wqf_rose@sina.com
  • Supported by:

    Basic Special Scientific Instruments of National Natural Science Foundation of China, No. 30527001*; General Project of National Natural Science Foundation of China, No. 3067087*; Key Science and Technology Planning Program of Chongqing, No. CSTC2009AB5003*

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Abstract:

BACKGROUND: Studies in recent years have found that the left ventricular papillary muscle plays an important role in ventricular arrhythmia.
OBJECTIVE: To observe the feasibility and safety of homemade ultrasound ablation catheter in animal left ventricular papillary muscle ablation with transthoracic echocardiography. 
METHODS: Nine ordinary mongrel dogs were collected and divided into two groups: control group (n=3) and ablation group (n=6). The carotid artery was separated with 3% pentobarbital sodium under sterile conditions, and implanted into the 8F arterial sheath was into to perform the mechanical ventilation, then the surface electrocardiogram was recorded. The abutting relationship between the catheter and papillary muscle was adjusted with transthoracic echocardiography image in order to keep the excellent contact between the catheter and papillary muscle. Then, 50 W/cm² acoustic intensity was applied to ablate the papillary muscle for 60 seconds.
RESULTS AND CONCLUSION: All the animals were normally survived during the observation period, the anatomical structure of papillary muscle was clearly displayed on the transthoracic echocardiography image, and the relative position and abutting relationship between catheter and papillary muscle could be confirmed with the transthoracic echocardiography image. After cutting the animal myocardium, the ablation lesions in the papillary muscle could be seen, which was white in central and light red around and a clear boundary with the surrounding normal myocardium. The clear boundary between ablation lesions and the surrounding tissues could be seen under light microscope, and many necrotic cardiomyocytes were observed in the ablation lesion which space was increased and infiltrated with red blood cells. No associated complications of ablation (such as thromboembolism, myocardial burned or perforation and cardiac rupture) were detected. The catheter could achieve the ablation of deep papillary muscle effectively and safely by transthoracic echocardiography real-time monitoring.

Key words: tissue construction, heart tissue construction, ultrasound, catheter, ablation, papillary muscle, ventricular tachycardia, National Natural Science Foundation of China

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