Zinc ions improve the stability of titanium implants under the condition of osteoporosis

doi:10.3969/j.issn.2095-4344.2013.29.006

Abstract

Abstract:

BACKGROUND: Zinc can improve bone reaction to titanium implants through regulation of osteoblast activity under the condition of osteoporosis.
OBJECTIVE: To study the zinc ions effect on improving the stability of titanium implants under the condition of osteoporosis.
METHODS: After removal of the ovary, 28 female Sprague-Dawley rats presented with osteoporosis symptoms for 12 weeks and then, they were randomly divided into two groups. Two kinds of titanium implants with (experimental group) or without zinc (control group) were inserted into the bilateral femoral metaphysis longitudinally in the ovariectomized rats, separately. Three rats were selected from each group for double fluorescence labeling examination. Mineral apposition rate was calculated at postoperatively 6 weeks. Another four rats were selected from each group for histomorphometry analysis at postoperatively 12 weeks. Biomechanical test was in the remaining rats in the two groups to calculate the maximum push-out force and maximum shear force at 12 weeks postoperatively.
RESULTS AND CONCLUSION: The double fluorescence labeling examination revealed that the mineral apposition rate was improved in the experimental group as compared with the control group (P < 0.05). New bone formation occurred in the two groups. However, larger new bone amount, higher bone area ratio and bone-to-implant contact were detected in the experimental group (P < 0.05 or P < 0.01). The maximum push-out force and maximum shear force were also superior in the experimental group to the control group (P < 0.05 or P < 0.01). These findings indicate that zinc ions are capable of promoting bone formation and improving implant fixation in ovariectomized rats.

Key words: biomaterials, tissue-engineered oral materials, zinc ions, titanium implants, osseointegration, osteoporosis, Sprague-Dawley rats, National Natural Science Foundation of China

CLC Number:

R318

Lin Wei, Li Xu-dong, Tang Kuang-yun, Hu Jing. Zinc ions improve the stability of titanium implants under the condition of osteoporosis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(29): 5296-5302.

Figures/Tables 7

References

[1] Mellado-Valero A,Ferrer-García JC,Calvo-Catalá J,et al. Implant treatment in patients with osteoporosis. Med Oral Patol Oral Cir Bucal.2010;15(1): e52-57.

[2] Bornstein MM,Cionca N,Mombelli A Systemic conditions and treatments as risks for implant therapy. Int J Oral Maxillofac Implants.2009;24 (Suppl): 12-27.

[3] van Steenberghe D,Jacobs R,Desnyder M,et al. The relative impact of local and endogenous patient-related factors on implant failure up to the abutment stage. Clin Oral Implants Res.2002;13: 617-622.

[4] Miettinen SS,Jaatinen J,Pelttari A,et al. Effect of locally administered zoledronic acid on injury-induced intramembranous bone regeneration and osseointegration of a titanium implant in rats. J Orthop Sci.2009;14: 431-436.

[5] Maïmoun L, Brennan TC,Badoud I, et al. Strontium ranelate improves implant osseointegration. Bone.2010;46: 1436-1441.

[6] Neale SD,Sabokbar A,Howie DW,et al. Macrophage colony-stimulating factor and interleukin-6 release by periprosthetic cells stimulates osteoclast formation and bone resorption. J Orthop Res.1999;17: 686-694.

[7] Marco F,Milena F,Gianluca G.et al. Peri-implant osteogenesis in health and osteoporosis. Micron.2005;36: 630-644.

[8] 袁旭敏,傅远飞,张富强,等. 羟基磷灰石涂层钛种植体结合强度及稳定性的研究进展[J].中国口腔种植学杂志,2007,12(3): 193-195.

[9] 陈海军,张安生,林均舟,等. 锌对钛种植体骨融合的影响[J].中国组织工程研究与临床康复,2011,15(51):9518-9522.

[10] Wang X,Ito A,Sogo Y,et al.Zinc-containing apatite layers on external fixation rods promoting cell activity. Acta Biomater. 2010;6:962-968.

[11] Wang Y,Zhang S,Zeng X,et al. Osteoblastic cell response on fluoridated hydroxyapatite coatings. Acta Biomater.2007;3: 191-197.

[12] Cai YL,Zhang JJ,Zhang S,et al.Osteoblastic cell response on fluoridated hydroxyapatite coatings: the effect of magnesium incorporation.Biomed Mater.2010;5: 054114.

[13] Oxlund BS,Ortoft G,Andreassen TT,et al.Low-intensity, high-frequency vibration appears to prevent the decrease in strength of the femur and tibia associated with ovariectomy of adult rats. Bone. 2003;32:69-77.

[14] Kurth AH,Eberhardt C,Muller S,et al.The bisphosphonate ibandronate improves implant integration in osteopenic ovariectomized rats. Bone. 2005;37:204-210.

[15] Gao Y,Luo E,Hu J, et al. Effect of combined local treatment with zoledronic acid and basic fibroblast growth factor on implant fixation in ovariectomized rats.Bone.2009;44: 225-232.

[16] Berzins A,Shah B,Weinans H,et al.Nondestructive measurements of implant-bone interface shear modulus and effects of implant geometry in pull-out tests.J Biomed Mater Res.1997;34:337-340.

[17] Avila G,Misch K,Galindo-Moreno P,et al.Implant surface treatment using biomimetic agents.Implant Dent.2009; 18: 17-26.

[18] Starck WJ,Epker BN.Failure of osseointegrated dental implants after diphosphonate therapy for osteoporosis: A case report. Int J Oral Maxillofac Implants.1995;10:74-78.

[19] Narai S,Nagahata S. Effects of alendronate on the removal torque of implants in rats with induced osteoporosis.Int J Oral Maxillofac Implants. 2003;18:218-223.

[20] Yang L,Perez-Amodio S,Barrere-de Groot FY,et al. The effects of inorganic additives to calcium phosphate on in vitro behavior of osteoblasts and osteoclasts. Biomaterials. 2010; 31:2976-2989.

[21] Kawamura H,Ito A,Miyakawa S,et al.Stimulatory effect of zinc-releasing calcium phosphate implant on bone formation in rabbit femora. J Biomed Mater Res.2000;50:184-190.

[22] Kempen DH,Lu L,Heijink A,et al. Effect of local sequential vegf and bmp-2 delivery on ectopic and orthotopic bone regeneration.Biomaterials. 2009;30:2816-2825.

[23] Cooper LF,Zhou Y,Takebe J,et al. Fluoride modification effects on osteoblast behavior and bone formation at tio2 grit-blasted c.P. Titanium endosseous implants. Biomaterials. 2006;27: 926-936.

[24] 王丹宁,赵宝红,张伟,等. 纯钛表面应用微弧氧化法制备不同浓度锌涂层对成骨细胞生物学特性影响的研究[J].口腔医学,2011, 31(10):577-581.

[25] 赵宝红,封伟,尚德浩,等. 纯钛表面微弧氧化法制备含钙锌涂层的抗菌性能研究[J].上海口腔医学,2012,21(3):266-269.