Glucocorticoid effects on adult hippocampal neural progenitor cells

doi:10.3969/j.issn.2095-4344.2013.19.016

Abstract

Abstract:

BACKGROUND: In vivo studies have shown that the high concentration of glucocorticoids can inhibit the proliferation of rat endogenous neural precursor cells. Neuron-glia antigen 2 proteoglycan-positive neural progenitor cells are the largest proliferating cell population in the mature central nervous system, which are pluripotent cells.
OBJECTIVE: To investigate the effects of glucocorticoid on the survival and proliferation of the neuron-glia antigen 2 proteoglycan-positive neural progenitor cells from in vitro cultured adult rat hippocampus.
METHODS: Neuron-glia antigen 2 proteoglycan-positive neural progenitor cells were primary cultured and sub-cultured. The passage 1 cells were treated with dexamethasone with the concentrations of 0, 0.1, 1, 10
and 100 μmol for 48 hours, and then the cell activities were determined by lactate dehydrogenase assay, the apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay, and the proliferation of the cells was identified by 5'-bromodeoxyuridine incorporation method.
RESULTS AND CONCLUSION: Dexamethasone with the concentrations of 1, 10 and 100 μmol could decrease the number of the neuron-glia antigen 2 proteoglycan-positive neural progenitor cells, increase the number of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling positive cells, and significantly reduce the number of BrdU positive cells. High concentration of glucocorticoid can decrease the number of the neuron-glia antigen 2 proteoglycan-positive neural progenitor cells by inhibiting the proliferation and inducing the apoptosis.

Key words: stem cells, stem cell culture and proliferation, glucocorticoid, neuron-glia antigen 2, neural progenitor cells, proliferation, apoptosis, stress, dexamethasone, hippocampus, primary culture, neurogenesis, provincial grants-supported paper

CLC Number:

R394.2

Yu Xiu-jun, Li Yi, Tai Li-wen, Chen Xiang. Glucocorticoid effects on adult hippocampal neural progenitor cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3521-3526.

Figures/Tables 3

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