Effects of intravenous transplantation of human umbilical cord blood mononuclear cells combined with perindopril on inflammatory response of acute myocardial infarction

doi:10.3969/j.issn.2095-4344.2013.19.010

Abstract

Abstract:

BACKGROUND: A recent study has indicated that angiotensin-converting enzyme inhibitor, perindopril, combined with transplantation of bone marrow-derived mesenchymal stem cells can reduce inflammatory response in the injured myocardium associated with enhanced improvement of cardiac function in an animal model of acute myocardial infarction.
OBJECTIVE: To observe the effects of intravenous transplantation of human umbilical cord blood mononuclear cells combined with perindopril on the inflammatory responses and pro-inflammatory cytokine interleukin-6 expression as well as the cardiac function in rabbits with acute myocardial infarction, and to investigate the possible protective mechanism of the combination treatment for acute myocardial infarction.
METHODS: The human umbilical cord blood mononuclear cells were obtained from healthy full-term delivery maternal cord blood. Sixty healthy rabbits were selected to establish the models of acute myocardial infarction, and the models were divided into four groups randomly: control group, perindopril group, simple transplantation group and combination group. Five rabbits were selected from each group to detect the cardiac function index of left ventricular ejection fraction and left ventricular fractional shortening with echocardiography at 1, 2 and 4 weeks after treatment. Hematoxylin-eosin staining was performed to observe the myocardial pathological changes and white blood cell count under light microscope. The protein expression of interleukin-6 in myocardial tissue was detected with immunohistochemistry. The number of green fluorescent protein-positive cells was observed under fluorescence microscope.
RESULTS AND CONCLUSION: Compared with the control group, left ventricular ejection fraction and left ventricular fractional shortening were significantly improved in the perindopril group, simple transplantation group and combination group at 1, 2 and 4 weeks respectively after the treatment (P < 0.05). The improvement of cardiac function index in simple transplantation group was higher than that in the perindopril group and most significantly in the combination group among the three treatment groups (P < 0.05). Compared with the control group, the number of white blood cells and the expression of interleukin-6 were decreased significantly in the perindopril group, simple transplantation group and combination group (P < 0.05). The number of white blood cells and the expression of interleukin-6 were lower in the simple transplantation group than in the perindopril group (P < 0.05), and lowest in the combination group among the three treatment groups (P < 0.05). Green fluorescent protein-positive cells were found to distribute in the peri-myocardial infarction area in the simple transplantation group and combination group at 1, 2 and 4 weeks after treatment, and the number of the green fluorescent protein-positive cells in the combination group was more than that in the simple transplantation group. The findings indicate that the combination of intravenous transplantation of human umbilical cord blood mononuclear cells and perindopril in the treatment of rabbits with acute myocardial infarction can increase the survival rate of transplanted cells, and further enhance the improvement of the heart function. The possible mechanism may be relate to the combination treatment, which can inhibit the local myocardial inflammation response and the expression of pro-inflammatory cytokine, interleukin-6, effectively.

Key words: stem cells, stem cell transplantation, myocardial infarction, human umbilical cord blood mononuclear cells, inflammation response, perindopril, intravenous transplantation, interleukin-6, angiotensin-converting enzyme inhibitors, myocardial tissue, myocardial cells, ventricular remodeling, stem cell photographs-containing paper

CLC Number:

R318

Deng Liu-xia, Yu Guo-long, Ai Qi, Yuan Chun-ju. Effects of intravenous transplantation of human umbilical cord blood mononuclear cells combined with perindopril on inflammatory response of acute myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3481-3487.

Figures/Tables 4

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